Proceedings Volume 2010 (format .pdf) - SimpBTH

106Table 1. Composition of culture media for Bulgarian Rhodiola rosea in vitro seeddevelopment, shoot multiplication, and rooting of plant regenerants.Media Concentration of phytoregulators and supplements [mg/l]variants Zea IAA NAA IBA 2,4-D GA 3G1 1 10G2 1 25G3 1 50G4 1 100Z-5 1,2 2.0 0.2 0.4V-1 1.0 1.0V-2 1.0 1.0V-3 2.0 1.0V-4 0.2 2.0 1.0V-5 0.5 1.0V-6 0.25 1.0V-7 0.1 1.0V-8 0.2 2.0 0.4Legend: 1 Sucrose is 30 g/l instead of 20 g/l; 2 Agar-agar is 0.4 % instead of 0.6 % as in other mediaRESULTS AND DISCUSSIONSDecontamination of Rhodiola rosea seeds.Ethyl alcohol treatment (Table 2, scheme 1) was insufficient for sterilization of‘old’ and ‘fresh’ seeds. The latter needed longer time treatment with bleach inaddition (scheme 2) for 100 % disinfection and high development ability (96 %).When scheme 3, 4 and 5 were used, the "old" seeds' decontamination was 100 %,however, without germination. Within 2-3 weeks bacterial contamination appearedaround the seeds. On the same three schemes (number 3, 4, and 5) low germinationrate (26 %, 23.7 %, and 22.5 %, respectively) was recorded for "fresh" seeds.Table 2. Decontamination efficiency of Rhodiola rosea L seeds.Plant material Scheme Cultivated Decontamination Development of seeds№ [ number] [%]and explants [%]Seeds – 1 year stored1 60 0 02 60 0 03 80 100 04 80 100 05 80 100 0Seeds – “fresh”1 60 0 02 100 100 96.03 100 100 26.04 80 100 23.75 80 100 22.5