Proceedings Volume 2010 (format .pdf) - SimpBTH

analysis of variance (ANOVA), and the means were separated using Duncan's testat P ≤ 0.05. For presentation, the data were inversely transformed. An index ofsomatic embryo-forming capacity (EFC) was used to evaluate cumulative effect ofthe mean SE number and frequency of regeneration, and it was calculated asfollows: EFC = (mean number of SE per regenerating section) x (% of regeneratingsections) / 100. For secondary regeneration from SE, we used an index ofsecondary somatic embryogenesis (SSE), calculated in the same manner as EFC.RESULTS AND DISCUSSIONInduction of SE regenerationIn this experiment we used highly responsive line 238-6-3-1, whichregenerated so efficiently that the whole root section's tissue turned into SE(Milojević et al. 2009). It showed early regeneration and produced no secondaryroots. For this reasons, the results were recorded at the end of the first subculture,so the procedure was less laborious.Fig. 1. - Somatic embryo regeneration from root sections of spinach cultivated on MSmedium with 20 µM NAA, 5 µM GA 3 and a) 0 mg/l hyg, b) 1 mg/l hyg, c) 2.5 mg/l hygand d) 7.5 mg/l hyg. a-d bar = 1 mm.Spinach root fragments cultivated on media with variable hyg concentrationproliferated, but the zones of proliferation were very limited and no voluminouscalli were formed (Fig. 1). The proliferations formed from roots cultivated on 0,114