Proceedings Volume 2010 (format .pdf) - SimpBTH

Proceeding of the 3 rd International Symposium“NEW RESEARCH IN BIOTECHNOLOGY” USAMV Bucharest, Romania, 2010SECTION I: AGRICULTURAL BIOTECHNOLOGYBEHAVIOUR OF SEVERAL POTATO (Solanum tuberosum L.) VARIETIESWITH DIFFERENT PROTEIN CONTENT TO THE POTATO TUBERNECROTIC RINGSPOT DISEASE AFTER PVY NTN INOCULATIONCARMEN LILIANA BĂDĂRĂU 1 , MĂRCULESCU ANGELA 2 ,N. COJOCARU, DAMŞA FLORENTINA 11 National Institute of Research and Development for Potato and Sugar Beet Brasov, Romania2Faculty of Food and Tourism, Transilvania University, Braşov, RomaniaCorresponding author: Carmen Liliana Badarau, National Institute of Research and Development for Potato andSugar Beet Brasov, 2 Fundaturii, 500470 Brasov, Romania, tel. 0040268476795, fax: 0040268476608,e-mail: carmen_badarau@yahoo.comAbstract. Obtaining good quality for potato seed impose the improvement of identification’stechniques of pathogen agents, knowing the biochemical composition of this kind of food, especiallythe components wich could affect its fitosanitary status. The goal of this research was to examine andevaluate the behaviour of 10 potato varieties to the desease caused by PVY NTN and the protein contentof these samples. Protein content of PVY NTN health plants from potato varieties of potato (Solanumtuberosum L.), wich differ in their susceptibility or resistance to virus, was compared. Another goal ofthis study was to elucidate the biochemical basis responsables for different reaction to infection withPVY NTN among the varieties: Christian, Romano, Roclas, Dacia, Tampa, Ostara, Agata, Hermes,Sante, Koretta. Excepting the cultivars Christian, Dacia and Sante, wich were very resistent andresistent to mechanical inoculation all the other varieties presented 62.5-100% infected plants. After3 months from harvesting, the frequency of tubers with symptoms was between 6.6-22.2% forvarieties Dacia, Agata, Romano, Roclas, Ostara and for Tampa, Koretta, Hermes varieties thispercentage was higher (60-95.8%). The protein content was significantly higher to the samplesresistents to PVY NTN inoculation. In this case (cv. Sante and Christian), after 3 months from harvest,the stored tubers didn’t have visible tuber necrotic ringspot disease symptoms.Keywords: potato virus Y, protein content, varieties, necrotic strain.1. INTRODUCTIONObtaining good quality for potato seed impose the improvement ofidentification’s techniques of pathogen agents, knowing the biochemicalcomposition of this kind of food, especially the components wich could affect itsfitosanitary status. Among the known pathogen agents infecting potato, the virusesproduce serious damages to potato quality. Potato virus Y (PVY) (Potyviride) isone of the most important potato’s viruses, (Solanum tuberosum L.) (Ragsdale et9