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2.Abdalla, A.E. and Roozen, J.P. (1999) Effect of plant extracts on the oxidative stability of sunfloweroil and emulsion, Food Chemistry, 64:323-3293.Andrej L. Dawidowics, Dorota Wianowska, Barbara Baraniak, (2006): The antioxidant propertiesof alcoholic extracts from Sambucus nigra L. (anioxidant properties of extracts). LWT. 39:308-3154.Ito N., Kakuda R., Yaoita Y., Kikichi M.(2006) - Five New Phenylethanoid Glycosides from theWhole Plants of Lamium purpureum L. - Chem Pharm. Bull, 54(12) 1705-1708,5.WAGNER H., BLADT S., Plant Drug Analysis, Second Edition, 1996, p195-245;6.Farmacopea Romana Editia X, p 259-260, p 10637. Huang, D., Ou, B., Prior, R.(2005),: The Chemistry behind Antioxidant Capacity Assays. In:Agric. Food. Chem. 53, 1841-185624
CO-TRANSFORMATION OF POTATO (SOLANUM TUBEROSUM L.)CV. DESIREE BY TWO SEPARATE T-DNAs CARRYINGORYZACYSTATIN GENESCINGEL ALEKSANDAR 1 , VINTERHALTER BRANKA 1 , VINTERHALTERDRAGAN 1 , MILOŠEVIĆ SNEŽANA 1 , SAVIĆ JELENA 1 , SMIGOCKI ANN 2 ,NINKOVIĆ SLAVICA 11 Institute for Biological Research “Siniša Stanković”, University of Belgrade, Bul Despota Stefana 142, 11060Belgrade, Republic of Serbia. E-mail: cingel@ibiss.bg.ac.rs2 USDA-ARS, Molecular Plant Pathology Laboratory, 10300 Baltimore Ave., Beltsville, MD 20705, USAKeywords: potato, Agrobacterium tumefaciens, co-transformation, oryzacystatin I,oryzacystatin IIABSTRACTIn an effort to develop plants with enhanced insect and pathogen resistance rice cystatin geneswere introduced into potato via Agrobacterium-mediated transformation. Plants of Solanumtuberosum L. cv. Desiree were co-transformed using the mixture method with two separateAgrobacterium tumefaciens EHA101 strains carrying oryzacystatin I (OCI) or oryzacystatin II (OCII)genes. PCR analysis indicated integration frequency of 38.0 ± 2.9% and 42.0 ± 5.8% for OCI andOCII genes, respectively, while co-transformation frequency of both rice cystatin genes was 20.0 ±2.9% of total number of analyzed transformants.1. INTRODUCTIONSterility and tetraploidy, together with a high degree of heterozygosity,significantly reduce the efficiency of conventional breeding as a methods forimproving potato genotypes. Agrobacterium-mediated gene transfer has appearedas promising technique to introduce heterologous genes into potato since it wasfirst reported by Ooms et al. (1986). A large number of agronomically useful geneshave already been transferred to potato. Most of these genes play role in aresistance to potato predators/pathogens (Chue et al., 2004; Naimov et al., 2001;Urwin et al., 2001; Lyapkova et al., 2001; Hefferon et al., 1997; Wierenga et al.,1996) and modification of important metabolic and physiological pathwayssuch as starch and sucrose synthesis (Edwards et al., 1999; Wolters et al., 1998).Co-transformation with multiple Agrobacterium strains carrying different T-DNA is mainly used to study the mechanism of T-DNA integration (De Neve etal., 1998; De Block et al., 1991), to facilitate selectable marker removal (Park etal., 2004; Vidal et al., 2003) or for multiple gene enhancements via geneticengineering (Li et al., 2009). This approach has not been widely used for potato,although it provides an effective method for rapidly stacking transgenes. Chang etal. (2002) reported a co-transformation efficiency of 24% for potato cv. RussetBurbank obtained using two plasmids cloned in separate A. tumefaciens LBA4404containing the same selectable marker. Using same approach Morris et al. (2006)25
Page 1 and 2: UNIVERSITY OF AGRONOMICAL SCIENCES
Page 3 and 4: SCIENTIFIC COMMITTEEProf. Dr. Petru
Page 5 and 6: CONTENTSECTION I: AGRICULTURAL BIOT
Page 7 and 8: SECTION IV: INDUSTRIAL AND ENVIRONM
Page 9 and 10: Proceeding of the 3 rd Internationa
Page 11 and 12: All the biological material (positi
Page 13 and 14: At harvesting, symptoms could be in
Page 15 and 16: Necrotic Disease (PTRND) induced by
Page 17 and 18: Eppendorfer, W., H., Eggum, B.,O.,
Page 19 and 20: Now Lamium genus from Lamiaceae fam
Page 21 and 22: extracts and rutin, hyperoside, chl
Page 23: Figure 3. Scavenging activity on DP
Page 27 and 28: transferred OCI and OCII genes were
Page 29 and 30: correspond to co-transformation fre
Page 31 and 32: EXPERIMENTS ON LASER RADIATION INFL
Page 33 and 34: formations. The average percentage
Page 35 and 36: Legend: Variants: V1 (5 minutes of
Page 37 and 38: We thank our partners at 4R OPTICS
Page 39 and 40: Regarding superior plants they are
Page 41 and 42: Fig. 2. The mechanism of the abscis
Page 43 and 44: In figure 3 it is presented the bio
Page 45 and 46: In the same time it has been proved
Page 47 and 48: egenerative lines of alfalfa, Wan e
Page 49 and 50: demonstrated direct regeneration fr
Page 51 and 52: (Knoll et al. 1997, Zhang and Zeeva
Page 53 and 54: the mean ± standard error. Six sam
Page 55 and 56: IMPACT OF PHOTOPERIOD ON SPINACH RE
Page 57 and 58: etween SD and LD response of the sa
Page 59 and 60: GA 3 plays an important role in spi
Page 61 and 62: INFLUENCE OF POLYETHYLENE GLYCOL (P
Page 63 and 64: enriched with 20 g / l sucrose, 8g
Page 65 and 66: If we compare the two varieties Roc
Page 67 and 68: For Roclas cultivar, the highest nu
Page 69 and 70: RESEARCH ON THE AVERAGE NUMBER OFPO
Page 71 and 72: two weeks before the harvest, were
Page 73 and 74: from minitubersfrom plantlets8.637.
Page 75 and 76:
If biological material influences t
Page 77 and 78:
BIOTECHNOLOGY OF ORGANIC CULTIVATIO
Page 79 and 80:
RESULTS AND DISCUSSIONAccording to
Page 81 and 82:
nitrogen sources, barley bran was t
Page 83 and 84:
In figure 5 the effects of inoculum
Page 85 and 86:
DORMANCY OF SEEDS AND HIS IMPORTANC
Page 87 and 88:
Dor. Large samples of grain were ha
Page 89 and 90:
Precip.23.06.200524.06.200525.06.20
Page 91 and 92:
certificates technologies. In the o
Page 93 and 94:
PROBLEMS RELATED TO RECULTIVATION O
Page 95 and 96:
Fig. 2 - Density of tests making in
Page 97 and 98:
The analysis made of the conditions
Page 99 and 100:
The mixtures are intense dynamic bi
Page 101 and 102:
Mixed growing of wintering pea and
Page 103 and 104:
RHODIOLA ROSEA L. IN VITRO CULTURES
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from Rila Mountain National Park of
Page 107 and 108:
In vitro seed germination and devel
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Along with rhizogenesis, shoot form
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4. Ganzera, M., Yayla, Y., Khan, I.
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1994), while timentin stimulated mo
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1 and 2.5 mg/l hyg were not necroti
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Nevertheless, we suggest stepwise i
Page 119 and 120:
SECTION II: BIOTECHNOLOGY IN VETERI
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The young pigs were fed in accordan
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LЕ39,8344,06100,25LЕ29,7944,793,6
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During the digestibility test daily
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- The use of fodder per unit of liv
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Table 1- The observational data for
Page 131 and 132:
Having arranged the treatment means
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difference between them is 0,78 and
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processes that occur during the win
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hundreds of fermenting experiences
Page 139 and 140:
In the case of spontaneous malolact
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With enzymeNo enzymesAstringencyRou
Page 143 and 144:
RESEARCHES ON THE BIOTECHNOLOGY POS
Page 145 and 146:
from Merlot grapes, with 224 g / l
Page 147 and 148:
lasted 12 days, while the variants
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case of the produces fermentation.
Page 151 and 152:
itself a protective factor against
Page 153 and 154:
THE INFLUENCE OF THE CHEMICAL COMPO
Page 155 and 156:
Sample witness Thiamine Magnesium s
Page 157 and 158:
Both the vitamines (thiamine) and t
Page 159 and 160:
This reaction takes place inside th
Page 161 and 162:
Sample witness Glycerol 2.5% Glycer
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The intensity of the connection can
Page 165 and 166:
HIGH NUTRITIVE BIOMASS OF EDIBLE AN
Page 167 and 168:
According to the purpose of this wo
Page 169 and 170:
Experiments were carried out in thr
Page 171 and 172:
Table 5. The sugar and total nitrog
Page 173 and 174:
STUDY OF THE CORRELATIONS BETWEEN G
Page 175 and 176:
The analyzed wheat was characterize
Page 177 and 178:
The lack of a significant relations
Page 179 and 180:
(phenotypically influenced) and glu
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DP700 integrator was used. Compound
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Of the esters were identified:•2-
Page 185 and 186:
Table 4 - Concentration of terpenes
Page 187 and 188:
SECTION IV: INDUSTRIAL AND ENVIRONM
Page 189 and 190:
Table 2. Variation of the compositi
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YoghurtAcerbityFresh butterVerdantF
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1991). This imposes the need of new
Page 195 and 196:
The crop weeds occurrence (mainly w
Page 197 and 198:
formation in the variant fertilized
Page 199 and 200:
gypsum from Sero-Cleaning Installat
Page 201 and 202:
DepthcmTable 1. Water soluble salts
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egulation in mixed filling of ashes
Page 205 and 206:
The aim of this paper is to use the
Page 207 and 208:
RESULTS AND DISCUSSIONPreliminary e
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M1 T1 A B T2 A BM2 T1 A B T2 A BM3
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As one can see in figure 4, the hig
Page 213 and 214:
STUDIES ON IMMOBILIZATION OF CELLUL
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Total immobilizatedprotein (%)80706
Page 217 and 218:
PHYTOREMEDIATION OF LEAD CONTAMINAT
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are placed through holes in the gro
Page 221 and 222:
To highlight phyto-remediation test
Page 223 and 224:
• The leaves have different abili
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include xylanase, endoxylanase, 1,4
Page 227 and 228:
In Taguchi technique, the variation
Page 229 and 230:
REFERENCES1.Amita, R.S., Shah, R.K.
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molecules, such as proteins, DNA, a
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• Quantitative determination of s
Page 235 and 236:
Antioxidant activityDPPH- Free radi
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quantitative determination of the f
Page 239 and 240:
ANTIOXIDANT BIOPRODUCT WITHIMMUNOMO
Page 241 and 242:
evaluations of the specific physica
Page 243 and 244:
TNF-α (pg/ml)100090080070060050040
Page 245 and 246:
THE ENVIRONMENTAL IMPACT OF VITICUL
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hydrocarbon components such as benz
Page 249 and 250:
RESULTS AND DISCUSSIONThe wine indu
Page 251 and 252:
CONCLUSIONIt takes 16 months under
Page 253 and 254:
VANILLIN RELEASE FROM AGAR MICROCAP
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only considering that an amount of
Page 257 and 258:
Fig 4. Surface vanillin (SV) versus
Page 259 and 260:
containing pumpkin extracts (1 ml/l
Page 261 and 262:
Table1 Effect of different types of
Page 263 and 264:
Table 3. Acclimatization of А. mon
Page 265 and 266:
SECTION V: FOOD SAFETYSENSORY ANALY
Page 267 and 268:
Depending on the product analysed,
Page 269 and 270:
Sensory analysis for organic versus
Page 271 and 272:
organic sample), colour (3,85 compa
Page 273 and 274:
Fig. 1.7. Organic (QAF) versus conv
Page 275 and 276:
CONTROL OF MICROBIAL GROWTH AND ENZ
Page 277 and 278:
determined in order to establish th
Page 279 and 280:
3,53log N (N = NTG/g apple slices)2
Page 281 and 282:
a - control; b - apple slices spray
Page 283 and 284:
DETERMINATION OF BIOCHEMICAL MARKER
Page 285 and 286:
Tabel 1. Characteristics of experim
Page 287 and 288:
The maximmal value has been recorde
Page 289 and 290:
Fig 3. Sensors responses for sample
Page 291 and 292:
AN OVERVIEW OF THE METHODS USED TO
Page 293 and 294:
After the sample extraction, the se
Page 295 and 296:
REFERENCES1. Bosset, J. O., Jeangro
Page 297 and 298:
CASE STUDY - METHODS AND FILM PACKA
Page 299 and 300:
Backbone Solution that provides bas
Page 301 and 302:
85 X 55 MM (CREDIT CARD SIZE) OR SM
Page 303 and 304:
the exposing of the entire range of
Page 305 and 306:
REFERENCES1. Agency - March 2002 -
Page 307 and 308:
keep the value of sensory and nutri
Page 309 and 310:
Material exposure to UV radiation w
Page 311 and 312:
Fig. 6. Influence of UV duration on
Page 313 and 314:
Table 3.4. The influence of PEF + U
Page 315 and 316:
SECTION VI: MISCELLANEOUSCOMPUTERIZ
Page 317 and 318:
The computer measures the encasing
Page 319 and 320:
measurements bulletin is typed by t
Page 321 and 322:
5. CONCLUSIONSThe implementation of
Page 323 and 324:
• the knowledge and the informati
Page 325 and 326:
The Agri-Food KMP will be extended,
Page 327 and 328:
REFERENCES1. Abell, A. A. and Oxbro
Page 329 and 330:
quicker translocation of the raw sa
Page 331 and 332:
with X1 [ a,b]such that M ( θ )( )
Page 333:
as it leads us in the right directi
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