Proceedings Volume 2010 (format .pdf) - SimpBTH

enriched with 20 g / l sucrose, 8g / l agar, 0.012 M PEG, and the upper layer waswithout PEG.In his experience was working with Romanian varieties: Christian andRoclas.Polyethylene glycol (PEG) with molecular weight of 6000, was usedas a simulator of water stress “in vitro”. Have been used two PEG concentrations(0.006 M, 0.012 M). The investigations were made both on a single layer ofpolyethylene glycol, and dual-layer (bottom layer: MS and PEG, and the upperlayer: MS).There have been measurements of the height of plantlets, number ofleaves, at six to eight weeks from inoculation to determine the response to drought.Fig.1. Plantlets with control mediumFig.2. Plantlets with PEGconcentrations, reduced heightand reduced number of leavesRESULTS AND DISCUSSIONResults on the influence of modified media supplemented withpolyethylene glycol on the height of plantlets obtained “in vitro”, for thevarieties testedAnalyzing the growth of plantlets (Table 2) for Christian cultivar on thefive culture media is observed that the presence of PEG-site to varying degrees ofwater stress simulated the growth of plantlets was inhibited. These results areshown in fig 3, where V1 is apparent that the control has not hampered the growthof plants, resulting from experiments height is 12.53 cm. The lowest height of theplantlets is observed for the variant with addition of 0.012 M PEG which stronglyinhibited growth, the resulting plantlets height was 2.27 cm.If we examine the variations in terms of number of layers used in culturemedia may indicate that variants 2 and 4 containing a single layer of culturemedium enriched with 0.006 M, 0.012 M PEG of PEG that have directly63