Proceedings Volume 2010 (format .pdf) - SimpBTH

RESEARCH ON THE AVERAGE NUMBER OFPOTATO MINITUBERS OBTAINED INDUSTRIAL SUBSTRATESANDREEA NISTOR 1) , GH. CAMPEANU 2) , N. ATANASIU 2) ,NICOLETA CHIRU 1) , DIANA KARÁCSONYI 1)1) Department of Vegetal Tissue Culture, National Institute of Research and Development for Potato and SugarBeet Brasov, Fundaturii no.2, Brasov, Romania; tican_andreea@yahoo.com2) University of Agronomical Sciences and Veterinary Medicine, Bucharest,RomaniaKey words: clay, perlite, plantlets, minitubersABSTRACTIn the years 2009 was a series of experiences polifactorials mounted in protected areasbelonging to the National Institute of Research and Development for Potato and Sugar beet Brasov.For culture substrates, soil was used as a witness, and perlite and clay balls were used as industrialsubstrates and biological material was composed of minitubers and plantlets obtained „in vitro”. Atthe end experiences were recorded minitubers number. Number of minitubers produced per plant,varied depending on different factors.The potato minituber production is the classical intermediate stageenabling field use of potato materials with an in vitro origin. Conventional methodsfor producing basic and certified potato seeds begins with the production of prebasicmaterial. Plants are produced in vitro using meristem culture and after anacclimatization period in greenhouse, are transferred to glasshouse where tubersare produced. The health risk at this stage depends in part upon the growthsubstrate used. Soil disinfestations generally is done with chemicals such as methylbromide, which eliminates both pathogenic and beneficial microorganisms. Thistechnique usually results in low yield of minitubers per plant, generally five or less,and is a serious constraint to the production (Daniels et al., 2000). Thedevelopment of more efficient and productive systems with lower environmentalimpact would improve the quality of the material used by the potato growers,directly affect the crop yields and reduce the production costs (Pereira and Fortes,2003; Pereira and Fortes, 2004). The use of hydroponic systems offers an excellentalternative to reduce the potential risks of root and tuber contamination by the soilpathogens and also for eliminating the need of chemical disinfectants, generallyvery harmful to the human health and environment (Medeiros et al., 2001; Pereiraand Fortes, 2003).By routing very precise, rigorous correlation of all factors of vegetation,based on high technological level of crops without soil substrate industry, isseeking obtaining minitubers of high biological value.69