Proceedings Volume 2010 (format .pdf) - SimpBTH

More documents

Recommendations

Info

13. Pueschel, A. K., Schwenkel, H. G., Winkelmann, T. (2003) Inheritance ability for regeneration viasomatic embryogenesis in Cyclamen persicum. Plant Cell Tiss. Org. Cult. Vol. 72, 1: 43-51.14. Rakshit, S., Rashid, Z., Sekhar, J.C., Fatma, T., Dass, S. (<strong>2010</strong>): Callus induction and whole plantregeneration in elite Indian maize (Zea mays L.) inbreds. Plant Cell Tiss. Org. Cult. Vol. 100, 1:31-3715. Wan, Y., Sorensen, E. L., Liang, G. H. (1988): Genetic control of in vitro regeneration in alfalfa(Medicago sativa L.). Euphytica Vol. 39, 1: 3-9.16. Xiao, X. G., Branchard, M. (1995): In vitro high frequency plant regeneration from hypocotyl androot segments of spinach by organogenesis. Plant Cell Tiss. Org. Cult. Vol. 42, 3: 239-244.17. Zdravković-Korać, S., Nešković, M. (1998): Organogenesis and somatic embryogenesis inSpinacia oleracea tissue culture. Arch. Biol. Sci., Belgrade Vol. 50, 3: 183-188.18. Zdravković-Korać, S., Nešković, M. (1999): Induction and development of somatic embryos fromspinach (Spinacia oleracea L.) leaf segments. Plant Cell Tiss. Org. Cult. Vol. 55, 2: 109-114.19. Zhang, H. X., Zeevaart, J.A.D. (1999): An efficient Agrobacterium tumefaciens-mediatedtrans<strong>format</strong>ion and regeneration system for cotyledons of spinach (Spinacia oleracea L.) Plant CellRep. Vol. 18, 7-8: 640-645.20. Zhang, C. L., Chen, D. F., Kubalakova, M., Zhang, J., Scott, N. W., Elliott, M. C., Slater, A.(2008): Efficient somatic embryogenesis in sugar beet (Beta vulgaris L.) breeding lines. Plant CellTiss. Org. Cult. Vol. 93, 2: 209-221.54
IMPACT OF PHOTOPERIOD ON SPINACH REGENERATIONCAPACITYJELENA MILOJEVIĆ 1 , LJILJANA TUBIĆ, SNEŽANA ZDRAVKOVIĆ-KORAĆ,DUŠICA ĆALIĆ-DRAGOSAVAC, BRANKA VINTERHALTER1 University of Belgrade, Institute for Biological Research "Siniša Stanković", Despot Stefan Blvd. 142, 11 060Belgrade, Republic of Serbia. E-mail: jelenamilojevic@ibiss.bg.ac.rsABSTRACTHigh variation in regeneration capacity of spinach at individual level masked the differences betweenregeneration responses in long days (LD) and short days (SD) in previous studies. In this study, weexposed explants isolated from the same individuals to both SD (8/16h light/dark period) and LD(16/8h light/dark period). Apical sections (1 cm) of seedling's lateral roots were used for theexperiment. Root sections were placed on solid medium with Murashige and Skoog's mineralsolution, 20 µM α-naphthaleneacetic acid and 5 µM gibberellic acid. A half of root sections isolatedfrom a single seedling has been exposed to SD, and the other half of root sections has been exposedto LD. In all of thirty seedlings used for the experiment, somatic embryos were noted in LD a fewweeks earlier than in SD. Both the frequency of regeneration and the mean number of somaticembryos were significantly higher in LD than in SD. Some of genotypes regenerated in LD only. Weassume that the process of somatic embryogenesis in spinach is triggered and controlled byphotoperiod.Keywords: Chenopodiaceae, photoperiod, somatic embryogenesis, Spinacea oleracea, tissueculture1. INTRODUCTIONSpinach is a vegetable crop used for human consumption, grown for leaves,which are rich in vitamins and other essential nutrients. At short days (SD) spinachplants force growth of vegetative organs, forming rosette, while in long days (LD)spinach plants bolt and flower. Eight continuous cycles of at least 12h of light arerequired for the induction of flowering (Ćulafić 1973), but spinach plants could beinduce to flower in SD conditions in response to different environmental stimuli,such as high temperature and egzogenously supplied GA 3 (Ćulafić and Nešković1980).In spite of this, almost all researches dealing with spinach tissue culture choseLD photoperiod for culture growth. In a preliminary study, we intended to cultivatespinich in SD, to prevent precocious flowering, but the induction of regenerationwas lower in SD than in LD, although the differences seemed statisticallyinsignificant. However, variations in regeneration response were observed amongdifferent spinach cultivars (Al-Khayri et al. 1991a; Komai et al. 1996; Knoll et al.1997), and even within population variations were reported for cultivars Nippon(Ishizaki et al. 2001) and Matador (Milojević et al. 2009). So, we assumed thathigh individual variations in regeneration response masked the differences causedby photoperiod. In this study, we assesed regeneration response of root sections of55
Page 1 and 2:
UNIVERSITY OF AGRONOMICAL SCIENCES
Page 3 and 4: SCIENTIFIC COMMITTEEProf. Dr. Petru
Page 5 and 6: CONTENTSECTION I: AGRICULTURAL BIOT
Page 7 and 8: SECTION IV: INDUSTRIAL AND ENVIRONM
Page 9 and 10: Proceeding of the 3 rd Internationa
Page 11 and 12: All the biological material (positi
Page 13 and 14: At harvesting, symptoms could be in
Page 15 and 16: Necrotic Disease (PTRND) induced by
Page 17 and 18: Eppendorfer, W., H., Eggum, B.,O.,
Page 19 and 20: Now Lamium genus from Lamiaceae fam
Page 21 and 22: extracts and rutin, hyperoside, chl
Page 23 and 24: Figure 3. Scavenging activity on DP
Page 25 and 26: CO-TRANSFORMATION OF POTATO (SOLANU
Page 27 and 28: transferred OCI and OCII genes were
Page 29 and 30: correspond to co-transformation fre
Page 31 and 32: EXPERIMENTS ON LASER RADIATION INFL
Page 33 and 34: formations. The average percentage
Page 35 and 36: Legend: Variants: V1 (5 minutes of
Page 37 and 38: We thank our partners at 4R OPTICS
Page 39 and 40: Regarding superior plants they are
Page 41 and 42: Fig. 2. The mechanism of the abscis
Page 43 and 44: In figure 3 it is presented the bio
Page 45 and 46: In the same time it has been proved
Page 47 and 48: egenerative lines of alfalfa, Wan e
Page 49 and 50: demonstrated direct regeneration fr
Page 51 and 52: (Knoll et al. 1997, Zhang and Zeeva
Page 53: the mean ± standard error. Six sam
Page 57 and 58: etween SD and LD response of the sa
Page 59 and 60: GA 3 plays an important role in spi
Page 61 and 62: INFLUENCE OF POLYETHYLENE GLYCOL (P
Page 63 and 64: enriched with 20 g / l sucrose, 8g
Page 65 and 66: If we compare the two varieties Roc
Page 67 and 68: For Roclas cultivar, the highest nu
Page 69 and 70: RESEARCH ON THE AVERAGE NUMBER OFPO
Page 71 and 72: two weeks before the harvest, were
Page 73 and 74: from minitubersfrom plantlets8.637.
Page 75 and 76: If biological material influences t
Page 77 and 78: BIOTECHNOLOGY OF ORGANIC CULTIVATIO
Page 79 and 80: RESULTS AND DISCUSSIONAccording to
Page 81 and 82: nitrogen sources, barley bran was t
Page 83 and 84: In figure 5 the effects of inoculum
Page 85 and 86: DORMANCY OF SEEDS AND HIS IMPORTANC
Page 87 and 88: Dor. Large samples of grain were ha
Page 89 and 90: Precip.23.06.200524.06.200525.06.20
Page 91 and 92: certificates technologies. In the o
Page 93 and 94: PROBLEMS RELATED TO RECULTIVATION O
Page 95 and 96: Fig. 2 - Density of tests making in
Page 97 and 98: The analysis made of the conditions
Page 99 and 100: The mixtures are intense dynamic bi
Page 101 and 102: Mixed growing of wintering pea and
Page 103 and 104: RHODIOLA ROSEA L. IN VITRO CULTURES
Page 105 and 106:
from Rila Mountain National Park of
Page 107 and 108:
In vitro seed germination and devel
Page 109 and 110:
Along with rhizogenesis, shoot form
Page 111 and 112:
4. Ganzera, M., Yayla, Y., Khan, I.
Page 113 and 114:
1994), while timentin stimulated mo
Page 115 and 116:
1 and 2.5 mg/l hyg were not necroti
Page 117 and 118:
Nevertheless, we suggest stepwise i
Page 119 and 120:
SECTION II: BIOTECHNOLOGY IN VETERI
Page 121 and 122:
The young pigs were fed in accordan
Page 123 and 124:
LЕ39,8344,06100,25LЕ29,7944,793,6
Page 125 and 126:
During the digestibility test daily
Page 127 and 128:
- The use of fodder per unit of liv
Page 129 and 130:
Table 1- The observational data for
Page 131 and 132:
Having arranged the treatment means
Page 133 and 134:
difference between them is 0,78 and
Page 135 and 136:
processes that occur during the win
Page 137 and 138:
hundreds of fermenting experiences
Page 139 and 140:
In the case of spontaneous malolact
Page 141 and 142:
With enzymeNo enzymesAstringencyRou
Page 143 and 144:
RESEARCHES ON THE BIOTECHNOLOGY POS
Page 145 and 146:
from Merlot grapes, with 224 g / l
Page 147 and 148:
lasted 12 days, while the variants
Page 149 and 150:
case of the produces fermentation.
Page 151 and 152:
itself a protective factor against
Page 153 and 154:
THE INFLUENCE OF THE CHEMICAL COMPO
Page 155 and 156:
Sample witness Thiamine Magnesium s
Page 157 and 158:
Both the vitamines (thiamine) and t
Page 159 and 160:
This reaction takes place inside th
Page 161 and 162:
Sample witness Glycerol 2.5% Glycer
Page 163 and 164:
The intensity of the connection can
Page 165 and 166:
HIGH NUTRITIVE BIOMASS OF EDIBLE AN
Page 167 and 168:
According to the purpose of this wo
Page 169 and 170:
Experiments were carried out in thr
Page 171 and 172:
Table 5. The sugar and total nitrog
Page 173 and 174:
STUDY OF THE CORRELATIONS BETWEEN G
Page 175 and 176:
The analyzed wheat was characterize
Page 177 and 178:
The lack of a significant relations
Page 179 and 180:
(phenotypically influenced) and glu
Page 181 and 182:
DP700 integrator was used. Compound
Page 183 and 184:
Of the esters were identified:•2-
Page 185 and 186:
Table 4 - Concentration of terpenes
Page 187 and 188:
SECTION IV: INDUSTRIAL AND ENVIRONM
Page 189 and 190:
Table 2. Variation of the compositi
Page 191 and 192:
YoghurtAcerbityFresh butterVerdantF
Page 193 and 194:
1991). This imposes the need of new
Page 195 and 196:
The crop weeds occurrence (mainly w
Page 197 and 198:
formation in the variant fertilized
Page 199 and 200:
gypsum from Sero-Cleaning Installat
Page 201 and 202:
DepthcmTable 1. Water soluble salts
Page 203 and 204:
egulation in mixed filling of ashes
Page 205 and 206:
The aim of this paper is to use the
Page 207 and 208:
RESULTS AND DISCUSSIONPreliminary e
Page 209 and 210:
M1 T1 A B T2 A BM2 T1 A B T2 A BM3
Page 211 and 212:
As one can see in figure 4, the hig
Page 213 and 214:
STUDIES ON IMMOBILIZATION OF CELLUL
Page 215 and 216:
Total immobilizatedprotein (%)80706
Page 217 and 218:
PHYTOREMEDIATION OF LEAD CONTAMINAT
Page 219 and 220:
are placed through holes in the gro
Page 221 and 222:
To highlight phyto-remediation test
Page 223 and 224:
• The leaves have different abili
Page 225 and 226:
include xylanase, endoxylanase, 1,4
Page 227 and 228:
In Taguchi technique, the variation
Page 229 and 230:
REFERENCES1.Amita, R.S., Shah, R.K.
Page 231 and 232:
molecules, such as proteins, DNA, a
Page 233 and 234:
• Quantitative determination of s
Page 235 and 236:
Antioxidant activityDPPH- Free radi
Page 237 and 238:
quantitative determination of the f
Page 239 and 240:
ANTIOXIDANT BIOPRODUCT WITHIMMUNOMO
Page 241 and 242:
evaluations of the specific physica
Page 243 and 244:
TNF-α (pg/ml)100090080070060050040
Page 245 and 246:
THE ENVIRONMENTAL IMPACT OF VITICUL
Page 247 and 248:
hydrocarbon components such as benz
Page 249 and 250:
RESULTS AND DISCUSSIONThe wine indu
Page 251 and 252:
CONCLUSIONIt takes 16 months under
Page 253 and 254:
VANILLIN RELEASE FROM AGAR MICROCAP
Page 255 and 256:
only considering that an amount of
Page 257 and 258:
Fig 4. Surface vanillin (SV) versus
Page 259 and 260:
containing pumpkin extracts (1 ml/l
Page 261 and 262:
Table1 Effect of different types of
Page 263 and 264:
Table 3. Acclimatization of А. mon
Page 265 and 266:
SECTION V: FOOD SAFETYSENSORY ANALY
Page 267 and 268:
Depending on the product analysed,
Page 269 and 270:
Sensory analysis for organic versus
Page 271 and 272:
organic sample), colour (3,85 compa
Page 273 and 274:
Fig. 1.7. Organic (QAF) versus conv
Page 275 and 276:
CONTROL OF MICROBIAL GROWTH AND ENZ
Page 277 and 278:
determined in order to establish th
Page 279 and 280:
3,53log N (N = NTG/g apple slices)2
Page 281 and 282:
a - control; b - apple slices spray
Page 283 and 284:
DETERMINATION OF BIOCHEMICAL MARKER
Page 285 and 286:
Tabel 1. Characteristics of experim
Page 287 and 288:
The maximmal value has been recorde
Page 289 and 290:
Fig 3. Sensors responses for sample
Page 291 and 292:
AN OVERVIEW OF THE METHODS USED TO
Page 293 and 294:
After the sample extraction, the se
Page 295 and 296:
REFERENCES1. Bosset, J. O., Jeangro
Page 297 and 298:
CASE STUDY - METHODS AND FILM PACKA
Page 299 and 300:
Backbone Solution that provides bas
Page 301 and 302:
85 X 55 MM (CREDIT CARD SIZE) OR SM
Page 303 and 304:
the exposing of the entire range of
Page 305 and 306:
REFERENCES1. Agency - March 2002 -
Page 307 and 308:
keep the value of sensory and nutri
Page 309 and 310:
Material exposure to UV radiation w
Page 311 and 312:
Fig. 6. Influence of UV duration on
Page 313 and 314:
Table 3.4. The influence of PEF + U
Page 315 and 316:
SECTION VI: MISCELLANEOUSCOMPUTERIZ
Page 317 and 318:
The computer measures the encasing
Page 319 and 320:
measurements bulletin is typed by t
Page 321 and 322:
5. CONCLUSIONSThe implementation of
Page 323 and 324:
• the knowledge and the informati
Page 325 and 326:
The Agri-Food KMP will be extended,
Page 327 and 328:
REFERENCES1. Abell, A. A. and Oxbro
Page 329 and 330:
quicker translocation of the raw sa
Page 331 and 332:
with X1 [ a,b]such that M ( θ )( )
Page 333:
as it leads us in the right directi
show all

Proceedings Volume 2010 (format .pdf) - SimpBTH

Create successful ePaper yourself

Delete template?

Save as template?