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Table 5. Survival of Rhodiola rosea plants regenerated in vitro after transfer to rootingsubstrates.Variant Rooting substrates (components andproportions)Air humidity[%]Survival[%]1 Soil 60 - 70 42 Soil 80 - 90 23 Peat 80 - 90 24 Perlite 60 - 70 Roots get rot5 Soil + perlite (3:1) 60 - 70 106 Soil + perlite (3:1) 80 - 90 127 Soil + peat (3:1) 60 - 70 108 Soil + peat (3:1) 80 - 90 229 Soil + peat + perlite (2:1:1) 60 - 70 3510 Soil + peat + perlite (2:1:1) 80 - 90 57CONCLUSIONSGolden root seed germination started on the 7 th day of cultivation and lasteduntil the 40 th day reaching from 37.5 % to 97.0 % depending on mediacomposition. Highest level of proliferation on Z-5 medium was observed in May-June, when the number of shoots per stem explant was 6.78. During cold seasonsmultiplication was relatively lower - 2.11 shoots per explant. The most efficientrhizogenesis concerning percentage of root induction (higher than 85 %) and rootlength (longer than 2 cm) was observed on the first scheme (seed germination onG1 medium, seedling explants development on Z-5 medium and induction ofrhizogenesisi on all rooting media (V1, V3-V8 variants). Best combination forplant adaptation was soil, peat and perlite in ratio of 2:1:1 and very high airhumidity (80 % to 90 %). In this environment, survival of plantlets is nearly 60 %and their further development in wild nature in the mountains was possible.ACKNOWLEDGEMENTSConsultation of Dr. L. Evstatieva from the Institute of Botany, BulgarianAcademy of Sciences is greatly appreciated. Research was supported by NationalScience Fund – Project CC MU 1201 (leader K. Tasheva) and ProjectBG051PO001/07/3.3-02/25/08REFERENCES1. Brown, R. P., Gerbarg, P. L., Ramazanov, Z. (2002): Rhodiola rosea: A Phytomedicinaloverview. Herbal Gram. Vol. 56, 40-52.2. Dimitrov, B., Tasheva, K., Zagorska, N., Evstatieva, L. (2003): In vitro cultivation of Rhodiolarosea L. Genetics and Breeding. Vol. 32, 1-2: 3-6.3. Farnsworth, N.R., Soejarto, D.D. (1991): Global importance of medicinal plants. TheConservation of Medicinal Plants. In: Akerele, O., Heywood, V., Synge, H. (Eds.), CambridgeUniversity Press, Cambridge. 25–52.110
4. Ganzera, M., Yayla, Y., Khan, I.A. (2000): Analysis of the marker copounds of Rh. rosea L.(golden root) by reversed phase high performance liquid chromatography. Arch. Pharm. Res. Vol.23, 4: 349-352.5. Ishmuratova, M.M. (1998): Clonal micropropagation of Rhodiola rosea L. and R. iremelicaBoriss. in vitro. Rastitel'nye Resursy. Vol. 34, 1:12-23, (in Russian)6. Ishmuratova, M.M. (2002): Effect of Rhodiola rosea Plant Extracts on the in vitro Developmentof Rhodiola rosea L. and Rhodiola iremelica Boriss. Explants. Biotekhnologiya. Vol. 6, 52-56, (inRussian).7. Kaftanat, V.N., Bodrug, M.V., Floryia, V.N. (1988): Enhanced multiplication of Rhodioloa roseain Moldova. <strong>Proceedings</strong> of the Second National Conference on Medicinal Botany, Kiev. 64. (inRussian)8. Kelly, G.S. (2001): Rhodiola rosea: a possible plant adaptogen. Altern Med Rev. Vol. 6, 3: 293-302.9. Kirichenko, E. B., Rudenko, C. C., Baglaii B. M., Masikevich, U. G. (1994): Rhodiola rosea invitro culture from leafs. Buletina GBS RAN, Moskva, Vol. 169, 50-54. (in Russian)10. Matthys, K. Julsing, Wim J. Quax, and Oliver Kayser. (2007): The engineering of medicinalplants: Prospects and Limitations of Medicinal Plant Biotechnology. Medicinal Plant Biotechnology.From Basic Research to Industrial Applications, In: Oliver Kayser and Wim J. Quax.(Eds.), WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim, 1-8.11. Murashige, T., Skoog, F. (1962): A revised medium for rapid growth and bio assays with tobaccotissue cultures. Physiol. Plant. Vol. 15, 473-497.12. Platikanov, S., Evstatieva, L. (2008): Introduction of Wild Golden Root (Rhodiola rosea L.) as aPotential Economic Crop in Bulgaria. Economic Botany. Vol. 62, 4: 621-627.13. Tasheva, K and Kosturkova, G. (<strong>2010</strong>): Bulgarian golden root in vitro cultures formicropropagation and reintroduction. Central European Journal of Biology (in press).14. Tripathi, L., and Tripathi, J. N. (2003): Role of biotechnology in medicinal plants. Trop. J.Pharm. Res., Vol. 2, 2: 243-253.15. Varabanova, K. (2002): Medicinal and aromatic plant diversity in Bulgaria – protection,collection, study, use and conservation. Report of a working group on medicinal and aromatic plants.First Meeting, Gozd Martuljek, Slovenia, 12-14 September, 27-32.16. Vitkova, A. and Evstatieva, L. (2000): Biodiversity of medicinal plants in the Rila National Park,In: Sakalian M. (Ed.), Biological diversity of the Rila National Park, Sofia, 79-116.17. Yin, W.B., Li, W., Du, G.S., Huang, Q.N. (2004): Studies on tissue culture of Tibetan Rhodiolarosea. Acta Bot. Boreal. Occident. Sin., Vol. 24, 1506-1510.111
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UNIVERSITY OF AGRONOMICAL SCIENCES
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SCIENTIFIC COMMITTEEProf. Dr. Petru
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CONTENTSECTION I: AGRICULTURAL BIOT
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SECTION IV: INDUSTRIAL AND ENVIRONM
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Proceeding of the 3 rd Internationa
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All the biological material (positi
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At harvesting, symptoms could be in
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Necrotic Disease (PTRND) induced by
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Eppendorfer, W., H., Eggum, B.,O.,
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Now Lamium genus from Lamiaceae fam
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extracts and rutin, hyperoside, chl
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Figure 3. Scavenging activity on DP
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CO-TRANSFORMATION OF POTATO (SOLANU
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transferred OCI and OCII genes were
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correspond to co-transformation fre
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EXPERIMENTS ON LASER RADIATION INFL
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formations. The average percentage
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Legend: Variants: V1 (5 minutes of
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We thank our partners at 4R OPTICS
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Regarding superior plants they are
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Fig. 2. The mechanism of the abscis
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In figure 3 it is presented the bio
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In the same time it has been proved
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egenerative lines of alfalfa, Wan e
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demonstrated direct regeneration fr
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(Knoll et al. 1997, Zhang and Zeeva
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the mean ± standard error. Six sam
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IMPACT OF PHOTOPERIOD ON SPINACH RE
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etween SD and LD response of the sa
Page 59 and 60: GA 3 plays an important role in spi
Page 61 and 62: INFLUENCE OF POLYETHYLENE GLYCOL (P
Page 63 and 64: enriched with 20 g / l sucrose, 8g
Page 65 and 66: If we compare the two varieties Roc
Page 67 and 68: For Roclas cultivar, the highest nu
Page 69 and 70: RESEARCH ON THE AVERAGE NUMBER OFPO
Page 71 and 72: two weeks before the harvest, were
Page 73 and 74: from minitubersfrom plantlets8.637.
Page 75 and 76: If biological material influences t
Page 77 and 78: BIOTECHNOLOGY OF ORGANIC CULTIVATIO
Page 79 and 80: RESULTS AND DISCUSSIONAccording to
Page 81 and 82: nitrogen sources, barley bran was t
Page 83 and 84: In figure 5 the effects of inoculum
Page 85 and 86: DORMANCY OF SEEDS AND HIS IMPORTANC
Page 87 and 88: Dor. Large samples of grain were ha
Page 89 and 90: Precip.23.06.200524.06.200525.06.20
Page 91 and 92: certificates technologies. In the o
Page 93 and 94: PROBLEMS RELATED TO RECULTIVATION O
Page 95 and 96: Fig. 2 - Density of tests making in
Page 97 and 98: The analysis made of the conditions
Page 99 and 100: The mixtures are intense dynamic bi
Page 101 and 102: Mixed growing of wintering pea and
Page 103 and 104: RHODIOLA ROSEA L. IN VITRO CULTURES
Page 105 and 106: from Rila Mountain National Park of
Page 107 and 108: In vitro seed germination and devel
Page 109: Along with rhizogenesis, shoot form
Page 113 and 114: 1994), while timentin stimulated mo
Page 115 and 116: 1 and 2.5 mg/l hyg were not necroti
Page 117 and 118: Nevertheless, we suggest stepwise i
Page 119 and 120: SECTION II: BIOTECHNOLOGY IN VETERI
Page 121 and 122: The young pigs were fed in accordan
Page 123 and 124: LЕ39,8344,06100,25LЕ29,7944,793,6
Page 125 and 126: During the digestibility test daily
Page 127 and 128: - The use of fodder per unit of liv
Page 129 and 130: Table 1- The observational data for
Page 131 and 132: Having arranged the treatment means
Page 133 and 134: difference between them is 0,78 and
Page 135 and 136: processes that occur during the win
Page 137 and 138: hundreds of fermenting experiences
Page 139 and 140: In the case of spontaneous malolact
Page 141 and 142: With enzymeNo enzymesAstringencyRou
Page 143 and 144: RESEARCHES ON THE BIOTECHNOLOGY POS
Page 145 and 146: from Merlot grapes, with 224 g / l
Page 147 and 148: lasted 12 days, while the variants
Page 149 and 150: case of the produces fermentation.
Page 151 and 152: itself a protective factor against
Page 153 and 154: THE INFLUENCE OF THE CHEMICAL COMPO
Page 155 and 156: Sample witness Thiamine Magnesium s
Page 157 and 158: Both the vitamines (thiamine) and t
Page 159 and 160: This reaction takes place inside th
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Sample witness Glycerol 2.5% Glycer
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The intensity of the connection can
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HIGH NUTRITIVE BIOMASS OF EDIBLE AN
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According to the purpose of this wo
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Experiments were carried out in thr
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Table 5. The sugar and total nitrog
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STUDY OF THE CORRELATIONS BETWEEN G
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The analyzed wheat was characterize
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The lack of a significant relations
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(phenotypically influenced) and glu
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DP700 integrator was used. Compound
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Of the esters were identified:•2-
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Table 4 - Concentration of terpenes
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SECTION IV: INDUSTRIAL AND ENVIRONM
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Table 2. Variation of the compositi
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YoghurtAcerbityFresh butterVerdantF
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1991). This imposes the need of new
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The crop weeds occurrence (mainly w
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formation in the variant fertilized
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gypsum from Sero-Cleaning Installat
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DepthcmTable 1. Water soluble salts
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egulation in mixed filling of ashes
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The aim of this paper is to use the
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RESULTS AND DISCUSSIONPreliminary e
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M1 T1 A B T2 A BM2 T1 A B T2 A BM3
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As one can see in figure 4, the hig
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STUDIES ON IMMOBILIZATION OF CELLUL
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Total immobilizatedprotein (%)80706
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PHYTOREMEDIATION OF LEAD CONTAMINAT
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are placed through holes in the gro
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To highlight phyto-remediation test
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• The leaves have different abili
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include xylanase, endoxylanase, 1,4
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In Taguchi technique, the variation
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REFERENCES1.Amita, R.S., Shah, R.K.
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molecules, such as proteins, DNA, a
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• Quantitative determination of s
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Antioxidant activityDPPH- Free radi
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quantitative determination of the f
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ANTIOXIDANT BIOPRODUCT WITHIMMUNOMO
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evaluations of the specific physica
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TNF-α (pg/ml)100090080070060050040
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THE ENVIRONMENTAL IMPACT OF VITICUL
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hydrocarbon components such as benz
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RESULTS AND DISCUSSIONThe wine indu
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CONCLUSIONIt takes 16 months under
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VANILLIN RELEASE FROM AGAR MICROCAP
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only considering that an amount of
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Fig 4. Surface vanillin (SV) versus
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containing pumpkin extracts (1 ml/l
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Table1 Effect of different types of
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Table 3. Acclimatization of А. mon
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SECTION V: FOOD SAFETYSENSORY ANALY
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Depending on the product analysed,
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Sensory analysis for organic versus
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organic sample), colour (3,85 compa
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Fig. 1.7. Organic (QAF) versus conv
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CONTROL OF MICROBIAL GROWTH AND ENZ
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determined in order to establish th
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3,53log N (N = NTG/g apple slices)2
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a - control; b - apple slices spray
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DETERMINATION OF BIOCHEMICAL MARKER
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Tabel 1. Characteristics of experim
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The maximmal value has been recorde
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Fig 3. Sensors responses for sample
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AN OVERVIEW OF THE METHODS USED TO
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After the sample extraction, the se
Page 295 and 296:
REFERENCES1. Bosset, J. O., Jeangro
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CASE STUDY - METHODS AND FILM PACKA
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Backbone Solution that provides bas
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85 X 55 MM (CREDIT CARD SIZE) OR SM
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the exposing of the entire range of
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REFERENCES1. Agency - March 2002 -
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keep the value of sensory and nutri
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Material exposure to UV radiation w
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Fig. 6. Influence of UV duration on
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Table 3.4. The influence of PEF + U
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SECTION VI: MISCELLANEOUSCOMPUTERIZ
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The computer measures the encasing
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measurements bulletin is typed by t
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5. CONCLUSIONSThe implementation of
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• the knowledge and the informati
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The Agri-Food KMP will be extended,
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REFERENCES1. Abell, A. A. and Oxbro
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quicker translocation of the raw sa
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with X1 [ a,b]such that M ( θ )( )
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as it leads us in the right directi
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