Proceedings Volume 2010 (format .pdf) - SimpBTH

RESULTSImmobilization yields of 90% were obtained when the ionic strength of theenzymatic solution was of 10-25 mM and the concentration of cellulases in thesolution was 5%, corresponding to 0.2 g of enzyme per g of adsorbent.Initialconcentration ofprotein(%)Table 1. Adsorption of cellulases on synthetic supportsProtein/supportratioLoading degree*Adsorption yield(%)XAD-7 XAD-8 XAD-7 XAD-81 1 : 10 0,090 0,075 90 922 1 : 8 0,100 0,090 85 903 1 : 5 0,170 0,165 83 834 1 : 4 0,200 0,180 80 845 1 : 3,5 0,225 0,200 79 805 1 : 2 0,225 0,200 „ „Immobilization yield(%)1008060402009080907590Concentration of citrate buffersolution, pH 5.5 (M)Fig 1. Influence of ionic strength of the buffer solution on immobilization yieldsMaximum irreversible immobilization yield by reticulation of the enzymewith glutaraldehyde was 75% and was obtained using a glutaraldehyde/proteinratio of 2.5/1.758060750,01 0,02 0,025 0,05 0,158XAD 7XAD 8214