Proceedings Volume 2010 (format .pdf) - SimpBTH

In figure 5 the effects of inoculum amounts added to the composts formycelia growing and mushroom fruit bodies producing can be noticed. Thus, thebest results were registered at the variants of 6% and 9% of inoculum (v/w) withinsignificant differences between them. The variant of 3% showed the lowestinfluence upon the mycelia growing. The control sample was established at 1%(v/w) inoculum added to each compost variants. All data are the means of tripledeterminations.Almost similar experiments concerning such techniques of mushroom cultivationwere made by Stamets (1993) as well as other researchers, but the culture substratawere different (Carlile & Watkinson, 1996; Moser, 1994; Moo-Young, 1993).The whole period of mushroom growing from the inoculation to the fruit bodyformation lasted between 30–60 d, depending on each fungal species used inexperiments. During the whole period of fruit body formation, the cultureparameters were set up and maintained at the following levels, depending on eachmushroom species: air temperature, 15–17 o C; the air flow volume, 5–6m 3 /h; airflow speed, 0.2–0.3 m/s; the relative moisture content, 80–85%, light intensity,500–1,000 luces for 8–10 h/d.The final fruit body production of these mushroom species used in experimentswas registered between 1.5–2.8 kg relative to 10 kg of composts made of vineyardand winery wastes.CONCLUSIONS1. The registered data revealed that by applying this biotechnology, the winery andvineyard wastes could be recycled as useful raw materials for culture compostpreparation in order to get edible mushrooms, the best results being obtained byusing the compost made from marc of grapes.2. Most suitable substratum for mycelia growing was prepared from marc ofgrapes, showing the highest influence upon the mycelia growing and freshmushroom production of 32–35 g%.3. From the tested nitrogen sources, barley bran was the most efficient upon themycelia growing and fruit mushroom producing at 35-40 g% fresh fungal biomassweight, being closely followed by rice bran at 25–30 g%.4. Among the various mineral sources examined, natural calcium carbonate(CaCO 3 ) from marine shells yielded the best mycelia growing as well as fungalbiomass production at 28-32 g% and for this reason it was registered as the mostappropriate mineral source being followed by natural gypsum (CaSO 4 · 2 H 2 O) at20-23 g%.5. Concerning the effects of inoculum amounts added to the composts for myceliagrowing and mushroom fruit bodies producing the best results were registered at83