Proceedings Volume 2010 (format .pdf) - SimpBTH

Pseudomonas(10 6 /ml). The seedbeds were set in March 2009, within completelynatural conditions. The mixture would be stirred from time to time to provideaerobic conditions for decomposition.Fig. 1 Mean air temperature and sum of precipitation in the year 2009120100806040T max°CT min°CT average°CHumidity %Precipitation mm200-20JanuaryFebruaryMartAprilMayJuneJulyAugustSeptemberOctoberNovemberDecemberESE »Radmilovac»: Altitude 130 m, Latitude 44°45´N, Longitude 20°35´EThere is a climatological station on the Faculty of Agriculture’s ExperimentalSchool Estate «Radmilovac», near which the experiment has been set. Since thedata for September and October are missing, data from the first-class meteorologystation, 5 km away from the vinyard, have been used instead. Data on averagemonthly air temperature values, relative air humidity and precipitations arepresented in Fig. 1. Seedbed samples were taken in Autumn 2009, Spring 2010,Autumn 2010, and then the analysis of microbiological presence was carried out.Microbiological characterization of samples was carried out through the use ofnutrient medium 0.1xTSA (for total microflora) and Fyodorov`s medium fordetermination of Azotobacter sp. The samples were prepared by the dilutionmethod. All experiments were performed in triplicate. The total microfloraincubation period was 5 days at 30°C and 2 days at 30°C for Azotobacter sp. Thepopulation densities were recorded as colony forming units (cfu) g -1 of drysamples. In autumn 2010, the following characteristics were determined: pH value,the content of calcium, humus, the total content of ammonium and nitrate forms ofnitrogen, the content of available phosporous and potassium as well as theproportion of carbon and nitrogen (C/N), all by means of the standard methods(Jakovljevic et al., 1998).248