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Immobilization yield (%)706060 60504037303229<strong>2010</strong>04 6 8 10 12 14 16Chitosan / Protein (g/g)Fig 4. Influence of the chitosan/protein ratio on the immobilization yieldsCONCLUSIONS• Maximum immobilization yields of 90% were obtained by adsorption;• In case of irreversible immobilization by reticulation with glutaraldehyde, themaximum immobilization yield was 75%• Maximum immobilization yield by entrapment was 60%• Experiments pointed out the positive effect of reticulation with glutaraldehydeand the efficiency of cellulases immobilization by adsorption on Amberlitepolymeric resins and by chitosan entrapmentREFERENCES1. Saville B., Khavkine M., Seetharam G., Marandi B., Zuo, Yong-Li, Appl. Biochem. Biotechnol.,113 (1-3), 251-259, 20042. Moran - Mirabal J., Anal. Chem. 79, 1109-1114, 20073. Esquisabel A., Hernandez R.M., Rodriguez Gascon A., Pedraz J.L., Methods in Biotechnology, 22,283-293, 20064. Takeuchi T., Makino K., Biotechnol. Bioeng., 29 (2), 160 – 164, 20045. Li C.Z., Yoshimoto M., Fukunaga K., Nakao K., Bioresource Technology, 98 (7), 1366-1372, 20076. Dourado F., Bastos M., Mota M., Gamma F.M., J. Biotechnol., 99, 121 -131, 20027. Ulijn R.V., Moore B.D., Janssen A.E.M., Halling P.J., J. Chem. Soc. Perkin II, 1024-1028, 20028. Afsahi B., Kazemi A., Khreilomoom A., Nejati S., Scientia Iranica, 14 (4), 379 – 383, 20079. Fernandes J.F.A , Halling P.J., Biotechnol. Prog., 18, 1455-1457, 200210. Ulijn R.V., Janssen A.E.M., Moore B.D., Halling P.J., Kelly S.M., Price N.C., Chem.Biochem., 3,1112-1116, 200211. Halling P.J., in K Drauz, H Waldmann and S Roberts, Eds, "Enzyme Catalysis in OrganicSynthesis: A Comprehensive Handbook", 2 nd ed., Wiley-VCH, Weinheim, 0-3527-29949-1, Vol I, pp259-285, 2002216
PHYTOREMEDIATION OF LEAD CONTAMINATED SOILSMARIA ICHIM, ADRIANA VISAN, RAMONA ENACHEInstitute of Biotechnology and Environmental Protection –S.C. BIOING S.A., Bucharest, RomaniaKey words: Morus sp., phytoremediation, leadABSTRACTIn the last decade, several researches have shown that in soils contaminated with heavymetals may reduce the content of chemical elements through phytoremediation.Basic and applied research has demonstrated unequivocally that certain plant species havegenetic potential to remove, degrade, metabolize, or immobilize a wide range of contaminants.Hyper-accumulator plants, which have ability to extract high concentrations of metals inthe soil in their tissues while retaining metabolic functions, are considered primary candidates forphytoremediation process.Despite regulatory measures adopted in many countries to limit contamination by Pb in theenvironment, it continues to be one of the most serious human and environmental hazards.Considering the fact that many Pb pollutants are essential to modern human life, soil contaminationwith Pb seems to fall in the near future.The article describes several models of plantation wood species belonging to the genusMorus. The phytoremediation process, metals are extracted from soil by plants of the genus Morushaving a high potential in this respect (plant extract heavy metals from soil and remedied biomass’squality of the soil).INTRODUCTIONLong-term application of fertilizers, sewage, pesticides and commercialwaste tailings containing heavy metals, resulted in surface contamination ofagricultural soils over the allowable limits, or on small areas or large surfaces.Techniques are currently available physico-chemical ex-situ extraction, usuallyexpensive. Moreover, they destroy the soil structure and leave it biologicallyinactive.The term phytoremediation is derived from the Greek word "phyton" -plant and from the Latin word "remediare" - to address and describe the system bywhich certain plants, interacting with soil organisms, can transform contaminantsinto harmless forms and sometimes valuable. This method is often used to improveland contaminated with heavy metals and toxic organic compounds.Extraction of heavy metals accumulation in plants (phyto-extraction) wassuggested as a strategy of in-situ remediation, mild to surface soils. Hyperaccumulatorplants are able to accumulate exceptionally high concentrations ofmetals in biomass without symptoms of phyto-toxicity.However, such hyper-accumulator plants have typically slow growing andproduce only small amounts of biomass, leading to a period of years the need fordecontamination of polluted sites. As an alternative, trees have been proposed due217
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UNIVERSITY OF AGRONOMICAL SCIENCES
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SCIENTIFIC COMMITTEEProf. Dr. Petru
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CONTENTSECTION I: AGRICULTURAL BIOT
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SECTION IV: INDUSTRIAL AND ENVIRONM
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Proceeding of the 3 rd Internationa
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All the biological material (positi
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At harvesting, symptoms could be in
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Necrotic Disease (PTRND) induced by
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Eppendorfer, W., H., Eggum, B.,O.,
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Now Lamium genus from Lamiaceae fam
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extracts and rutin, hyperoside, chl
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Figure 3. Scavenging activity on DP
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CO-TRANSFORMATION OF POTATO (SOLANU
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transferred OCI and OCII genes were
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correspond to co-transformation fre
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EXPERIMENTS ON LASER RADIATION INFL
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formations. The average percentage
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Legend: Variants: V1 (5 minutes of
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We thank our partners at 4R OPTICS
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Regarding superior plants they are
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Fig. 2. The mechanism of the abscis
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In figure 3 it is presented the bio
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In the same time it has been proved
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egenerative lines of alfalfa, Wan e
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demonstrated direct regeneration fr
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(Knoll et al. 1997, Zhang and Zeeva
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the mean ± standard error. Six sam
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IMPACT OF PHOTOPERIOD ON SPINACH RE
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etween SD and LD response of the sa
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GA 3 plays an important role in spi
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INFLUENCE OF POLYETHYLENE GLYCOL (P
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enriched with 20 g / l sucrose, 8g
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If we compare the two varieties Roc
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For Roclas cultivar, the highest nu
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RESEARCH ON THE AVERAGE NUMBER OFPO
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two weeks before the harvest, were
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from minitubersfrom plantlets8.637.
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If biological material influences t
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BIOTECHNOLOGY OF ORGANIC CULTIVATIO
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RESULTS AND DISCUSSIONAccording to
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nitrogen sources, barley bran was t
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In figure 5 the effects of inoculum
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DORMANCY OF SEEDS AND HIS IMPORTANC
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Dor. Large samples of grain were ha
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Precip.23.06.200524.06.200525.06.20
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certificates technologies. In the o
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PROBLEMS RELATED TO RECULTIVATION O
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Fig. 2 - Density of tests making in
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The analysis made of the conditions
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The mixtures are intense dynamic bi
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Mixed growing of wintering pea and
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RHODIOLA ROSEA L. IN VITRO CULTURES
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from Rila Mountain National Park of
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In vitro seed germination and devel
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Along with rhizogenesis, shoot form
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4. Ganzera, M., Yayla, Y., Khan, I.
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1994), while timentin stimulated mo
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1 and 2.5 mg/l hyg were not necroti
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Nevertheless, we suggest stepwise i
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SECTION II: BIOTECHNOLOGY IN VETERI
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The young pigs were fed in accordan
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LЕ39,8344,06100,25LЕ29,7944,793,6
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During the digestibility test daily
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- The use of fodder per unit of liv
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Table 1- The observational data for
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Having arranged the treatment means
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difference between them is 0,78 and
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processes that occur during the win
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hundreds of fermenting experiences
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In the case of spontaneous malolact
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With enzymeNo enzymesAstringencyRou
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RESEARCHES ON THE BIOTECHNOLOGY POS
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from Merlot grapes, with 224 g / l
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lasted 12 days, while the variants
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case of the produces fermentation.
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itself a protective factor against
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THE INFLUENCE OF THE CHEMICAL COMPO
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Sample witness Thiamine Magnesium s
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Both the vitamines (thiamine) and t
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This reaction takes place inside th
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Sample witness Glycerol 2.5% Glycer
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The intensity of the connection can
Page 165 and 166: HIGH NUTRITIVE BIOMASS OF EDIBLE AN
Page 167 and 168: According to the purpose of this wo
Page 169 and 170: Experiments were carried out in thr
Page 171 and 172: Table 5. The sugar and total nitrog
Page 173 and 174: STUDY OF THE CORRELATIONS BETWEEN G
Page 175 and 176: The analyzed wheat was characterize
Page 177 and 178: The lack of a significant relations
Page 179 and 180: (phenotypically influenced) and glu
Page 181 and 182: DP700 integrator was used. Compound
Page 183 and 184: Of the esters were identified:•2-
Page 185 and 186: Table 4 - Concentration of terpenes
Page 187 and 188: SECTION IV: INDUSTRIAL AND ENVIRONM
Page 189 and 190: Table 2. Variation of the compositi
Page 191 and 192: YoghurtAcerbityFresh butterVerdantF
Page 193 and 194: 1991). This imposes the need of new
Page 195 and 196: The crop weeds occurrence (mainly w
Page 197 and 198: formation in the variant fertilized
Page 199 and 200: gypsum from Sero-Cleaning Installat
Page 201 and 202: DepthcmTable 1. Water soluble salts
Page 203 and 204: egulation in mixed filling of ashes
Page 205 and 206: The aim of this paper is to use the
Page 207 and 208: RESULTS AND DISCUSSIONPreliminary e
Page 209 and 210: M1 T1 A B T2 A BM2 T1 A B T2 A BM3
Page 211 and 212: As one can see in figure 4, the hig
Page 213 and 214: STUDIES ON IMMOBILIZATION OF CELLUL
Page 215: Total immobilizatedprotein (%)80706
Page 219 and 220: are placed through holes in the gro
Page 221 and 222: To highlight phyto-remediation test
Page 223 and 224: • The leaves have different abili
Page 225 and 226: include xylanase, endoxylanase, 1,4
Page 227 and 228: In Taguchi technique, the variation
Page 229 and 230: REFERENCES1.Amita, R.S., Shah, R.K.
Page 231 and 232: molecules, such as proteins, DNA, a
Page 233 and 234: • Quantitative determination of s
Page 235 and 236: Antioxidant activityDPPH- Free radi
Page 237 and 238: quantitative determination of the f
Page 239 and 240: ANTIOXIDANT BIOPRODUCT WITHIMMUNOMO
Page 241 and 242: evaluations of the specific physica
Page 243 and 244: TNF-α (pg/ml)100090080070060050040
Page 245 and 246: THE ENVIRONMENTAL IMPACT OF VITICUL
Page 247 and 248: hydrocarbon components such as benz
Page 249 and 250: RESULTS AND DISCUSSIONThe wine indu
Page 251 and 252: CONCLUSIONIt takes 16 months under
Page 253 and 254: VANILLIN RELEASE FROM AGAR MICROCAP
Page 255 and 256: only considering that an amount of
Page 257 and 258: Fig 4. Surface vanillin (SV) versus
Page 259 and 260: containing pumpkin extracts (1 ml/l
Page 261 and 262: Table1 Effect of different types of
Page 263 and 264: Table 3. Acclimatization of А. mon
Page 265 and 266: SECTION V: FOOD SAFETYSENSORY ANALY
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Depending on the product analysed,
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Sensory analysis for organic versus
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organic sample), colour (3,85 compa
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Fig. 1.7. Organic (QAF) versus conv
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CONTROL OF MICROBIAL GROWTH AND ENZ
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determined in order to establish th
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3,53log N (N = NTG/g apple slices)2
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a - control; b - apple slices spray
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DETERMINATION OF BIOCHEMICAL MARKER
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Tabel 1. Characteristics of experim
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The maximmal value has been recorde
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Fig 3. Sensors responses for sample
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AN OVERVIEW OF THE METHODS USED TO
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After the sample extraction, the se
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REFERENCES1. Bosset, J. O., Jeangro
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CASE STUDY - METHODS AND FILM PACKA
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Backbone Solution that provides bas
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85 X 55 MM (CREDIT CARD SIZE) OR SM
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the exposing of the entire range of
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REFERENCES1. Agency - March 2002 -
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keep the value of sensory and nutri
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Material exposure to UV radiation w
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Fig. 6. Influence of UV duration on
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Table 3.4. The influence of PEF + U
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SECTION VI: MISCELLANEOUSCOMPUTERIZ
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The computer measures the encasing
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measurements bulletin is typed by t
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5. CONCLUSIONSThe implementation of
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• the knowledge and the informati
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The Agri-Food KMP will be extended,
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REFERENCES1. Abell, A. A. and Oxbro
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quicker translocation of the raw sa
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with X1 [ a,b]such that M ( θ )( )
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as it leads us in the right directi
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