Proceedings Volume 2010 (format .pdf) - SimpBTH

Table 3. Acclimatization of А. montana in depending from size of plants and roots.Glassified groups of Heigth of plants, Length of roots, Number ofplantscmcm transplantedSurvival ofplantlets,%plantsG1 - Short 1.0-1.5 0.5-1.0 30 66.7G2 - Medium 1.5-2.0 1.0-1.5 40 85G3 - Tall 2.0-2.5 1.5-2.0 26 46.1Note: Peat: perlite: coco (2:1:1)Fig 1. In vitro and in vivo cultivation of A. montana: a) In vitro rooted plants at 0.5 mg/lIBA; b) In vitro rooted plants at 0.5 mg/l NAA; c) In vitro rooted plants at 0.5 mg/l IAA;d), e) and f) Ex vitro acclimatized plants; g) A. montana during flowering stage.CONCLUSIONIBA was found to be the best auxin for rizogenesis of A. montana, followed byNAA and IAA. The optimum rooting efficiency for shoots (100%) as well as the bestroot number per shoot (5.0) were obtained on ½ MS medium supplemented with 0.5mg/l IBA. Relatively low concentration of IBA promoted both the frequency ofshoots forming roots and the number of roots/shoot. Adding NAA to the nutrientmedium yielded the longest roots and shoots. The data suggest that different mixturesubstrates had favourable effect on the survival of plants during acclimatization. The263