Proceedings Volume 2010 (format .pdf) - SimpBTH

M1 T1 A B T2 A BM2 T1 A B T2 A BM3 T1 A B T2 A BFig3. Cell elongation after T1 (two hours) and T2 (four hours) from the start of theexperiment, cells were stained with acridine orange and viewed with epifluorescencemicroscopy (x1000); A - original microphotographs; B – total count of bacteria fromoriginal microphotographs using CellC software.In figure 3A there are microphotographs of bacteria from M1, M2, M3 and infigure 3 digital analysis of pannel A using CellC software. We adjustcontrast/brightness of digital image with ImageJ software, then analyse measure ofgraticula and set the calibration bar to determine correctly the length of eachbacteria. Image processing methods used guarantee that all images are analyzedusing the same criteria, and therefore results between different images arecomparable. Images for analysis were done with a Sony DSC-P200, 7.2megapixels digital camera). Brightness and contrast were adjusted for the firstimage and kept unchanged throughout the image acquisition procedure.The images (1600 by 1200 pixels, 256 dpi) were acquired at 50x magnification andstored as 543-KB JPG files. Additional images acquired at 100x magnificationwere used to verify that measurements of individual cells.Because CellC software included graphical user interface, and the batch processingmode enables fast and convenient processing of hundreds of cell images, we easillyenumerate bright cells on a dark background (epifluorescence). We also used two209