13. Leple, J., Bonade-Bottino, M., Augustin, S., Pilate, G., Letan, V., Delplanque, A., Cornu, D.,Jouanin, L. (1995): Toxicity to Chrysomela tremulae (Coleoptera: Chrysomelidae) of transgenicpoplars expressing a cysteine proteinase inhibitor. Mol. Breed. 1: 319-328.14. Li, F., Wu. S., Chen, T., Zhang, J., Wang, H., Guo, W., Zhang, T.(2009): Agrobacterium-mediatedco-trans<strong>format</strong>ion of multiple genes in upland cotton. Plant Cell Tiss Organ Cult. 97:225–23515. Lyapkova, N.S., Loskutova, N.A., Maisuryan, A.N. (2001): Transformed potato plants carrying thegene of the antifungal peptide of Amaranthus caudatus. Appl. Biochem. Microbiol. 37: 301-305.16. Morris, L.W., Ducreuxa, J.M.L., Fraserb, D.P., Millam, S., Taylora, A.M. (2006): Engineeringketocarotenoid biosynthesis in potato tubers. Metabolic Engineering 8: 253–26317. Murashige, T., Skoog, F. (1962): A revised medium for rapid growth and bioassays with tobaccotissues cultures. Phys. Plant, 15: 473-479.18. Naimov, S., Weemen-Hendriks, M., Dukiandjiev, S., De Maagd, R.A. (2001): Bacillusthuringiensis _-endotoxin Cry1 hybrid proteins with increased activity against the Coloradopotato beetle. Appl. Environ. Microbiol. 67: 5328-5330.19. Ninković, S., Miljuš-Djukić, J., Radović, S., Maksimović, V., Lazarević, J., Vinterhalter, B.,Nešković, M., Smigocki, A. (2007): Phytodecta fornicata Bruggemann resistance mediated byoryzacystatin II proteinase inhibitor transgene. Plant Cell Tissue Organ Cult. 91: 289-294.20. Ooms, G., Bossen, M.E., Burrell, M.M., Karp, A. (1986): Genetic manipulation in potato withAgrobacterium rhizogenes. Potato Res. 29: 367-379.21. Park, J., Lee, Y.K., Kang, B.K., Chung, WI. (2004): Co-trans<strong>format</strong>ion using a negative selectablemarker gene for the production of selectable marker gene-free transgenic plants. Theor ApplGenet 109:1562–1567.22. Ribeiro, E., Pereira, J.G., Galvan, T.L., Picano, M.C., Picoli, E.A.T., da Silva, D.J.H., Fari, M.G.,Otoni, W.C. (2006): Effect of eggplant transformed with oryzacystatin gene on Myzus persicaeand Macrosiphum euphorbiae. J. Appl. Entomol. 130(2) 84-90.23. Samac, D.A., Smigocki, A.C. (2003): Expression of Oryzacystatin I and II in Alfalfa IncreasesResistance to the Root-Lesion Nematode. Phytopatology, 93(7): 799-804.24. Uchimiya, H., Hirochika, H., Hashimoto, H., Hara, A., Masuda, T., Kasumimoto, T., Harada, H.,Ikeda, J.E., Yoshioka, M. (1986): Coexpression and inheritance of foreign genes in transformantsobtained by direct DNA trans<strong>format</strong>ion of tobacco protoplasts. Mol. Gen. Genet. 205: 1-8.25. Urwin, P.E., Troth, K.M,, Zubko, E.I., Atkinson, H.J. (2001): Efective transgenic resistance toGlobodera palida in potato field trials. Mol. Breed. 8: 95-101.26. Vidal, J.R., Kikkert, J.R., Wallace, P.G., Reisch, B.I. (2003): High-efficiency biolistic cotrans<strong>format</strong>ionand regeneration of ‘Chardonnay’ (Vitis vinifera L.) containing npt-II andantimicrobial peptide genes. Plant Cell Rep 22:252–260.27. Visser, R.G.F., Jacobsen, E., Hesselingmeinders, A., Schans, M.J., Witholt, B., Feenstra, W.J.(1989): Trans<strong>format</strong>ion of homozygous diploid potato with an Agrobacterium tumefaciens binaryvector system by adventitious shoot regeneration on leaf and stem segments. Plant Mol. Biol. 12:329-33.28. Webb, K.J., Osifo, E.O., Henshaw, G.G. (1983): Shoot regeneration from leaflet discs of sixcultivars of potato (Solanum tuberosum subsp. tuberosum). Plant Sci. Lett. 30: 1-8.29. Wenzler, H., Mignery, G., May, G., Park, W. (1989): A rapid and efficient trans<strong>format</strong>ion methodfor the production of large numbers of transgenic potato plants. Plant Sci. 63: 79-85.30. Wierenga,,J.M., Norris, D.L., Whalon, M.E. (1996): Stage specific mortality of Colorado potatobeetle (Coleoptera: Chrysomelidae) feeding on transgenic potatoes. J. Entomol. 89: 1047-1052.31. Wolters, A.M.A., Janssen, E.M., Rozeboom-Schippers, M.G.M., Jacobsen, E., Visser, R.G.F.(1998): Composition of endogenous alleles can influence the level of antisense inhibition ofgranule-bound starch synthase gene expression in tetraploid potato plants. Mol. Breed. 4: 343-358.30
EXPERIMENTS ON LASER RADIATION INFLUENCE ON THEDEVELOPMENT AND GROWTH OF SEEDLINGS OF EGGPLANT(SOLANUM MELONGENA SP.-VARIETY" DRAGAICA")DANAILA GUIDEA SILVANA M.¹, ESOFINA RISTICI², BURNICHIFLOAREA³,MARIAN RISTICI², PETRU NICULIŢĂ¹,DRAGHICI MIHAELA¹, DUMITRU CATALIN¹¹U.Ş.A.M.V. Bucuresti – Faculty of Biotechnology ;²4R OPTICS SRL. Bucuresti³Research and Development Station for Vegetables Growing BuzauABSTRACTAdditional red light illumination produced by laser diodes in continuous and differentexposure times, was applied to „eggplant” seeds (Solanum melongena sp.) from Romanian type“Dragaica”.Sets of seeds were irradiated once in the first day of the experiment at different energydoses by changing exposure time. Thus, four dry seed lots were irradiated at different doses during theperiod from 2009 and <strong>2010</strong> corresponding variants V1-V4 (5-20 minutes). The experimental resultswere analyzed in parallel with a control group of seeds that did not treated with red laser diodes.The capacity of germination and the growth rithm were studied for all seeds of eggplantSolanum melongena-variety "Dragaica by the determining the germination rates every two days for 3weeks.Results have shown a percentage of germination higher than that for the control group ofseeds (75%) for all repetitions of variant V4 (95%), with the time exposure of 20 minutes (1.53 joules /cm ²). In the treatment was used red light produced by 19 laser diodes.To seedlings of eggplants resulted from seeds irradiated in an experimental series S1-<strong>2010</strong>were made observations on the dynamics of <strong>format</strong>ion of true leaves at 14 and 30 days after the onsetof germination, compared with control group seedlings.Key words: laser radiation, germination seeds, plant growth, eggplant.INTRODUCTIONBodies known plant can not grow in the absence of light. In fact, light iselectromagnetic radiation with wavelengths in the range 400 nm - 750nm. In nonprotectedareas, light reaching the plants is generated by the sun, while in protectedareas light is generated by sources with certain spectral characteristics (Anghel, S.,et al., 1999; Călugăreanu M., et al., 1999;).Light is to plants, both a source of energy and in<strong>format</strong>ion. It is a sourceof energy for photosynthesis and a source of in<strong>format</strong>ion for photoperiodism (night/ day), phototropism (light direction) and photomorphogenesis (quantity andquality of light). The light in the spectral range 640 nm - 660nm is very importantconsidering that chlorophyll absorbs strongly in this range. Red field - about 660-670nm, is important to the absorption bands of chlorophylls, a 'and' b ' ( Quail,P.,H.,1991).31
- Page 1 and 2: UNIVERSITY OF AGRONOMICAL SCIENCES
- Page 3 and 4: SCIENTIFIC COMMITTEEProf. Dr. Petru
- Page 5 and 6: CONTENTSECTION I: AGRICULTURAL BIOT
- Page 7 and 8: SECTION IV: INDUSTRIAL AND ENVIRONM
- Page 9 and 10: Proceeding of the 3 rd Internationa
- Page 11 and 12: All the biological material (positi
- Page 13 and 14: At harvesting, symptoms could be in
- Page 15 and 16: Necrotic Disease (PTRND) induced by
- Page 17 and 18: Eppendorfer, W., H., Eggum, B.,O.,
- Page 19 and 20: Now Lamium genus from Lamiaceae fam
- Page 21 and 22: extracts and rutin, hyperoside, chl
- Page 23 and 24: Figure 3. Scavenging activity on DP
- Page 25 and 26: CO-TRANSFORMATION OF POTATO (SOLANU
- Page 27 and 28: transferred OCI and OCII genes were
- Page 29: correspond to co-transformation fre
- Page 33 and 34: formations. The average percentage
- Page 35 and 36: Legend: Variants: V1 (5 minutes of
- Page 37 and 38: We thank our partners at 4R OPTICS
- Page 39 and 40: Regarding superior plants they are
- Page 41 and 42: Fig. 2. The mechanism of the abscis
- Page 43 and 44: In figure 3 it is presented the bio
- Page 45 and 46: In the same time it has been proved
- Page 47 and 48: egenerative lines of alfalfa, Wan e
- Page 49 and 50: demonstrated direct regeneration fr
- Page 51 and 52: (Knoll et al. 1997, Zhang and Zeeva
- Page 53 and 54: the mean ± standard error. Six sam
- Page 55 and 56: IMPACT OF PHOTOPERIOD ON SPINACH RE
- Page 57 and 58: etween SD and LD response of the sa
- Page 59 and 60: GA 3 plays an important role in spi
- Page 61 and 62: INFLUENCE OF POLYETHYLENE GLYCOL (P
- Page 63 and 64: enriched with 20 g / l sucrose, 8g
- Page 65 and 66: If we compare the two varieties Roc
- Page 67 and 68: For Roclas cultivar, the highest nu
- Page 69 and 70: RESEARCH ON THE AVERAGE NUMBER OFPO
- Page 71 and 72: two weeks before the harvest, were
- Page 73 and 74: from minitubersfrom plantlets8.637.
- Page 75 and 76: If biological material influences t
- Page 77 and 78: BIOTECHNOLOGY OF ORGANIC CULTIVATIO
- Page 79 and 80: RESULTS AND DISCUSSIONAccording to
- Page 81 and 82:
nitrogen sources, barley bran was t
- Page 83 and 84:
In figure 5 the effects of inoculum
- Page 85 and 86:
DORMANCY OF SEEDS AND HIS IMPORTANC
- Page 87 and 88:
Dor. Large samples of grain were ha
- Page 89 and 90:
Precip.23.06.200524.06.200525.06.20
- Page 91 and 92:
certificates technologies. In the o
- Page 93 and 94:
PROBLEMS RELATED TO RECULTIVATION O
- Page 95 and 96:
Fig. 2 - Density of tests making in
- Page 97 and 98:
The analysis made of the conditions
- Page 99 and 100:
The mixtures are intense dynamic bi
- Page 101 and 102:
Mixed growing of wintering pea and
- Page 103 and 104:
RHODIOLA ROSEA L. IN VITRO CULTURES
- Page 105 and 106:
from Rila Mountain National Park of
- Page 107 and 108:
In vitro seed germination and devel
- Page 109 and 110:
Along with rhizogenesis, shoot form
- Page 111 and 112:
4. Ganzera, M., Yayla, Y., Khan, I.
- Page 113 and 114:
1994), while timentin stimulated mo
- Page 115 and 116:
1 and 2.5 mg/l hyg were not necroti
- Page 117 and 118:
Nevertheless, we suggest stepwise i
- Page 119 and 120:
SECTION II: BIOTECHNOLOGY IN VETERI
- Page 121 and 122:
The young pigs were fed in accordan
- Page 123 and 124:
LЕ39,8344,06100,25LЕ29,7944,793,6
- Page 125 and 126:
During the digestibility test daily
- Page 127 and 128:
- The use of fodder per unit of liv
- Page 129 and 130:
Table 1- The observational data for
- Page 131 and 132:
Having arranged the treatment means
- Page 133 and 134:
difference between them is 0,78 and
- Page 135 and 136:
processes that occur during the win
- Page 137 and 138:
hundreds of fermenting experiences
- Page 139 and 140:
In the case of spontaneous malolact
- Page 141 and 142:
With enzymeNo enzymesAstringencyRou
- Page 143 and 144:
RESEARCHES ON THE BIOTECHNOLOGY POS
- Page 145 and 146:
from Merlot grapes, with 224 g / l
- Page 147 and 148:
lasted 12 days, while the variants
- Page 149 and 150:
case of the produces fermentation.
- Page 151 and 152:
itself a protective factor against
- Page 153 and 154:
THE INFLUENCE OF THE CHEMICAL COMPO
- Page 155 and 156:
Sample witness Thiamine Magnesium s
- Page 157 and 158:
Both the vitamines (thiamine) and t
- Page 159 and 160:
This reaction takes place inside th
- Page 161 and 162:
Sample witness Glycerol 2.5% Glycer
- Page 163 and 164:
The intensity of the connection can
- Page 165 and 166:
HIGH NUTRITIVE BIOMASS OF EDIBLE AN
- Page 167 and 168:
According to the purpose of this wo
- Page 169 and 170:
Experiments were carried out in thr
- Page 171 and 172:
Table 5. The sugar and total nitrog
- Page 173 and 174:
STUDY OF THE CORRELATIONS BETWEEN G
- Page 175 and 176:
The analyzed wheat was characterize
- Page 177 and 178:
The lack of a significant relations
- Page 179 and 180:
(phenotypically influenced) and glu
- Page 181 and 182:
DP700 integrator was used. Compound
- Page 183 and 184:
Of the esters were identified:•2-
- Page 185 and 186:
Table 4 - Concentration of terpenes
- Page 187 and 188:
SECTION IV: INDUSTRIAL AND ENVIRONM
- Page 189 and 190:
Table 2. Variation of the compositi
- Page 191 and 192:
YoghurtAcerbityFresh butterVerdantF
- Page 193 and 194:
1991). This imposes the need of new
- Page 195 and 196:
The crop weeds occurrence (mainly w
- Page 197 and 198:
formation in the variant fertilized
- Page 199 and 200:
gypsum from Sero-Cleaning Installat
- Page 201 and 202:
DepthcmTable 1. Water soluble salts
- Page 203 and 204:
egulation in mixed filling of ashes
- Page 205 and 206:
The aim of this paper is to use the
- Page 207 and 208:
RESULTS AND DISCUSSIONPreliminary e
- Page 209 and 210:
M1 T1 A B T2 A BM2 T1 A B T2 A BM3
- Page 211 and 212:
As one can see in figure 4, the hig
- Page 213 and 214:
STUDIES ON IMMOBILIZATION OF CELLUL
- Page 215 and 216:
Total immobilizatedprotein (%)80706
- Page 217 and 218:
PHYTOREMEDIATION OF LEAD CONTAMINAT
- Page 219 and 220:
are placed through holes in the gro
- Page 221 and 222:
To highlight phyto-remediation test
- Page 223 and 224:
• The leaves have different abili
- Page 225 and 226:
include xylanase, endoxylanase, 1,4
- Page 227 and 228:
In Taguchi technique, the variation
- Page 229 and 230:
REFERENCES1.Amita, R.S., Shah, R.K.
- Page 231 and 232:
molecules, such as proteins, DNA, a
- Page 233 and 234:
• Quantitative determination of s
- Page 235 and 236:
Antioxidant activityDPPH- Free radi
- Page 237 and 238:
quantitative determination of the f
- Page 239 and 240:
ANTIOXIDANT BIOPRODUCT WITHIMMUNOMO
- Page 241 and 242:
evaluations of the specific physica
- Page 243 and 244:
TNF-α (pg/ml)100090080070060050040
- Page 245 and 246:
THE ENVIRONMENTAL IMPACT OF VITICUL
- Page 247 and 248:
hydrocarbon components such as benz
- Page 249 and 250:
RESULTS AND DISCUSSIONThe wine indu
- Page 251 and 252:
CONCLUSIONIt takes 16 months under
- Page 253 and 254:
VANILLIN RELEASE FROM AGAR MICROCAP
- Page 255 and 256:
only considering that an amount of
- Page 257 and 258:
Fig 4. Surface vanillin (SV) versus
- Page 259 and 260:
containing pumpkin extracts (1 ml/l
- Page 261 and 262:
Table1 Effect of different types of
- Page 263 and 264:
Table 3. Acclimatization of А. mon
- Page 265 and 266:
SECTION V: FOOD SAFETYSENSORY ANALY
- Page 267 and 268:
Depending on the product analysed,
- Page 269 and 270:
Sensory analysis for organic versus
- Page 271 and 272:
organic sample), colour (3,85 compa
- Page 273 and 274:
Fig. 1.7. Organic (QAF) versus conv
- Page 275 and 276:
CONTROL OF MICROBIAL GROWTH AND ENZ
- Page 277 and 278:
determined in order to establish th
- Page 279 and 280:
3,53log N (N = NTG/g apple slices)2
- Page 281 and 282:
a - control; b - apple slices spray
- Page 283 and 284:
DETERMINATION OF BIOCHEMICAL MARKER
- Page 285 and 286:
Tabel 1. Characteristics of experim
- Page 287 and 288:
The maximmal value has been recorde
- Page 289 and 290:
Fig 3. Sensors responses for sample
- Page 291 and 292:
AN OVERVIEW OF THE METHODS USED TO
- Page 293 and 294:
After the sample extraction, the se
- Page 295 and 296:
REFERENCES1. Bosset, J. O., Jeangro
- Page 297 and 298:
CASE STUDY - METHODS AND FILM PACKA
- Page 299 and 300:
Backbone Solution that provides bas
- Page 301 and 302:
85 X 55 MM (CREDIT CARD SIZE) OR SM
- Page 303 and 304:
the exposing of the entire range of
- Page 305 and 306:
REFERENCES1. Agency - March 2002 -
- Page 307 and 308:
keep the value of sensory and nutri
- Page 309 and 310:
Material exposure to UV radiation w
- Page 311 and 312:
Fig. 6. Influence of UV duration on
- Page 313 and 314:
Table 3.4. The influence of PEF + U
- Page 315 and 316:
SECTION VI: MISCELLANEOUSCOMPUTERIZ
- Page 317 and 318:
The computer measures the encasing
- Page 319 and 320:
measurements bulletin is typed by t
- Page 321 and 322:
5. CONCLUSIONSThe implementation of
- Page 323 and 324:
• the knowledge and the informati
- Page 325 and 326:
The Agri-Food KMP will be extended,
- Page 327 and 328:
REFERENCES1. Abell, A. A. and Oxbro
- Page 329 and 330:
quicker translocation of the raw sa
- Page 331 and 332:
with X1 [ a,b]such that M ( θ )( )
- Page 333:
as it leads us in the right directi