Proceedings Volume 2010 (format .pdf) - SimpBTH

Table1 Effect of different types of auxins in ½ MS medium on rhizogenesis of А. MontanaType of auxinConcentrationof auxins, mg/lRooting plants,%Number of rootsper plant(х ± SE)Root length,cm(х ± SE)Control 0 50 1.2±0.30 1.3±0.18IBA 0.5 100 5.0±0.16 1.5±0.111 100 3.7±0.38 1.8±0.142 100 3.5±0.31 2.4±0.38NAA 0.5 100 4.1±0.28 1.8±0.151 90 3.8±0.27 2.0±0.222 90 2.2±0.34 2.4±0.42IAA 0.5 85 2.5±0.41 1.0±0.151 80 2.1±0.44 1.3±0.342 70 1.9±0.37 1.8±0.372. Effect of Naphthalene Acetic Acid (NAA) on rooting of A. montana: The datarelated to the effect of the auxins on rooting of A. montana showed that rootingpercentage is high (100%) when the shoots were cultured on ½ MS supplementedwith 0.5 mg/l NAA. The average number of roots per plant was 4.1 and average rootlength was 1.8 cm (Figure 1b). When 1mg/l or 2 mg/l NAA were used, 90% rootedplants with average number of roots 3.8 and 2.2, respectively, were recorded (Figure1b). Root number was affected also by NAA concentration. Increasing NAAconcentration decreased gradually number of roots. On the other hand, root lengthincreased with increasing NAA concentration. Root length ranged from 1.8 to 2.4cm. After treatment with NAA no significant differences in shoot growth wereobserved. During four weeks of culture, roots were light green and thicker. Butiuc-Keul and Deliu (2001) found that MS medium containing NAA (5.3 µM), 2iP (5.0µM) and maize extract (1.0 ml/l) resulted in best root induction.3. Effect of Iindole-3-Acetic Acid (IAA) on rooting of A. montana: The effect ofIAA on in vitro root formation of A. montana were estimated as root percentage, rootnumber and length (Table 1). Of the tested IAA concentrations, the best treatment forin vitro rooting was ½ MS with 0.5 mg/l IAA yielding the highest values of rootformation (85%) with highest number of roots per shoot (2.5) (Figure 1c). The resultsshow that the addition of IAA (1 and 2 mg/l) led to lower percentage of rooting (80%and 70%, respectively) compared with the culture medium containing 0.5 mg/l IAA.The root length ranged from 1.0 to 1.8 cm. IAA at high concentration (2 mg/l)resulted in the highest root length (1.8 cm). The in vitro grown shoots cultured in allmedia containing IAA gave thin roots, which teared easily from basis of plant whenwere transffered in adaptation conditions.261