Proceedings Volume 2010 (format .pdf) - SimpBTH

C( S/ N) C1 = 41,185 C1 – 122,65( S/ N) C2 = 40,635 C2 – 113,13( S/ N) C3 = 42,453 C3 – 141,56Optim level 3D( S/ N) D1 = 40,867 D1 – 120,85( S/ N) D2 = 40,455 D2 – 118,32( S/ N) D3 = 42,003 D3 – 133,85Optim level 3The average values of S/N ratios of the four control factors at each of thelevels are shown above, and from which the levels corresponding to the highestS/N ratio values were chosen for each parameter representing the optimumcondition. It is clear that the optimum levels are: A2, B3, C3, D3.In addition to S/N analysis, main effects of the process parameters on themean response are also analyzed. The mean response refers to the average value ofthe quality characteristic for each factor at different levels. Thus the average valuesof the enzymatic activity for each factor at the three levels have been calculated.The mean response analysis also indicates the same optimum level of theparameters (A2, B3, C3, and D3) as was obtained in S/N ratio analysis.228Optimum recipe: Glycerol 1,5%Wheat bran 7%Corn steep liquor 2%Ammonium sulfate 0,4%Magnesium sulphate 0,05%Monopotassium phosphate 0,1%CONCLUSIONSOptimization medium components was performed via Taguchi’s parameterdesign method. An L9 orthogonal array was used to accommodate four controlfactors and each with three levels for the experimental plan.The factorial plan considered points out the microorganism potential to usethe carbon source glycerol 1.5 % and wheat bran 7 % , and that the organicnitrogen source - corn extract - is best used at 2 % ratio; the variants werereproducible for all of the 3 optimizing experiments.These experiments provided basic information to improve the efficiency ofxylanase production and supported the analysis of the main effect of eachconstituent of the medium.