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Table 2. - Secondary somatic embryogenesis from spinach somatic embryos cultured onMS medium with 20 µM NAA and 5 µM GA 3 and variable levels of hygromycin (hyg).hyg(mg/l)Frequency ofsecondary somaticembryogenesis (%)Mean ± SENo. of secondarysomatic embryos perprymary embryoMean ± SESSENecroticexplants%0 87.5 ± 0.9 ab 4.36 ± 0.05 b 3.81 6.1 ± 1.6 c1 95.3 ± 1.8 a 6.29 ± 0.05 a 5.99 8.9 ± 2.4 c2.5 81.7 ± 0.2 ab 5.35 ± 0.06 a 4.37 6.1 ± 1.6 c5 75.9 ± 1.9 ab 2.73 ± 0.05 bc 2.07 30.2 ± 1.7 bc7.5 69.1 ± 2.6 b 2.11 ± 0.04 c 1.46 53.4 ± 3.3 b10 57.8 ± 1.1 bc 0.71 ± 0.03 d 0.41 98.1 ± 0.6 a12.5 31.2 ± 2.2 cd 0.21 ± 0.02 de 0.06 100 a15 25.0 ± 1.8 cd 0.11 ± 0.01 de 0.03 99.2 ± 0.8 a20 7.4 ± 1.3 d 0.01 ± 0 e 0 100 aData indicate the mean ± standard error. Two samples, each with 15 subsamples (n = 30), were usedper treatment. Treatments denoted by the same letter are not significantly different (P ≤ 0.05)according to Duncan's test. SSE - secondary somatic embryogenesis, SE - standard error.116Fig. 2. - Secondary somatic embryo regeneration from somatic embryos cultured on MSmedium supplemented with 20 µM NAA, 5 µM GA 3 and a) 0 mg/l hyg, b) 1 mg/l hygand c) 7.5 mg/l hyg. a-c bar = 1 cm.This study suggests 10 mg/l hyg as suitable level for selection of transgeniccells, at least under these experimental conditions. However, complete absence ofproliferation was attained at 20 mg/l hyg. Chin et al. (2009) used this concentrationto select transformed cells from calli induced from spinach leaf fragments.
Nevertheless, we suggest stepwise increase of hyg concentration, to benefit thestimulative effect of hyg on SE regeneration, as non-transformed spinich SE wereintolerant to hyg and starting from 10 mg/l showed almost 100 % necrosis.The effect of some penicillins was explained by a discovery of Holford andNewbury (1992), who found that these substances break down to givephysiologically active levels of the auxin phenylacetic acid. However, to the best ofour knowledge, this kind of research was not reported for hygromycin.CONCLUSIONSWe reported here on stimulation of SE regeneration by lower doses of hyg. Theresults indicated 10 mg/l hyg as suitable level for selection, as the regeneration ofnon-transformed tissue and its multiplication were completely inhibited at thisconcentration. This study will be useful for genetic transformation of thisresponsive spinach line.ACKNOWLEDGEMENTSThis work was supported by the Ministry of Science and TechnologicalDevelopment of the Republic of Serbia, Contract No. 143026B.REFERENCES1. Chin, D.P., Bao, J. H., Mii, M. (2009): Transgenic spinach plants produced by Agrobacteriummediatedmethod based on the low temperature-dependent high plant regeneration ability of leafexplants. Plant Biotechnol. Vol. 26, 2: 243-248.2. Costa, M. G. C., Nogueira, F. T. S., Figueira, M. L., Otoni, W. C., Brommonschenkel, S. H.,Cecon, P. R. (2000): Influence of the antibiotic timentin on plant regeneration of tomato(Lycopersicon esculentum Mill.) cultivars. Plant Cell Rep. Vol. 19, 3: 327-332.3. Da Silva Mendes, A. F., Cidade, L. C., De Oliveira, M. L. P., Otoni, W. C., Soares-Filho, W. D.S., Costa, M. G. C. (2009): Evaluation of novel beta-lactam antibiotics in comparison to cefotaximeon plant regeneration of Citrus sinensis L. Osb. Plant Cell Tiss. Org. Cult. Vol. 97, 3: 331-336.4. Eady, C.C., Lister, C.E. (1998): A comparison of four selective agents for use with Allium cepa L.immature embryos and immature embryo-derived cultures. Plant Cell Rep. Vol. 18, 1-2: 117-121.5. Eapen, S., George, L. (1990): Influence of phytohormones, carbohydrates, aminoacids, growthsupplements and antibiotics on somatic embryogenesis and plant differentiation in finger millet. PlantCell Tiss. Org. Cult. Vol. 22, 2: 87-93.6. Holford, P., Newbury, H. J. (1992): The effect of antibiotics and their breakdown products on thein vitro growth of Antirrhinum majus. Plant Cell Rep. Vol. 11, 2: 93-96.7. Humara, J.M., Ordas, R.J. (1999): The toxicity of antibiotics and herbicides on in vitroadventitious shoot formation on Pinus pinea L. cotyledons. In Vitro Cell Dev. Biol. Plant. Vol. 35,4: 339-343.8. Knoll, K. A., Short, K. C., Curtis, I. S., Power, J. B., Davey, J. B. (1997): Shoot regeneration fromcultured root explants of spinach (Spinacia oleracea L.): a system for Agrobacterium transformation.Plant Cell Rep. Vol. 17, 2: 96-101.9. Mathias, R.J., Boyd, L.A. (1986): Cefotaxime stimulates callus growth, embryogenesis andregeneration in hexaploid bread wheat (Triticum aestivum L. em. thell). Plant Sci. Vol. 46, 3:217-223.117
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UNIVERSITY OF AGRONOMICAL SCIENCES
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SCIENTIFIC COMMITTEEProf. Dr. Petru
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CONTENTSECTION I: AGRICULTURAL BIOT
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SECTION IV: INDUSTRIAL AND ENVIRONM
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Proceeding of the 3 rd Internationa
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All the biological material (positi
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At harvesting, symptoms could be in
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Necrotic Disease (PTRND) induced by
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Eppendorfer, W., H., Eggum, B.,O.,
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Now Lamium genus from Lamiaceae fam
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extracts and rutin, hyperoside, chl
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Figure 3. Scavenging activity on DP
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CO-TRANSFORMATION OF POTATO (SOLANU
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transferred OCI and OCII genes were
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correspond to co-transformation fre
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EXPERIMENTS ON LASER RADIATION INFL
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formations. The average percentage
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Legend: Variants: V1 (5 minutes of
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We thank our partners at 4R OPTICS
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Regarding superior plants they are
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Fig. 2. The mechanism of the abscis
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In figure 3 it is presented the bio
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In the same time it has been proved
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egenerative lines of alfalfa, Wan e
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demonstrated direct regeneration fr
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(Knoll et al. 1997, Zhang and Zeeva
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the mean ± standard error. Six sam
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IMPACT OF PHOTOPERIOD ON SPINACH RE
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etween SD and LD response of the sa
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GA 3 plays an important role in spi
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INFLUENCE OF POLYETHYLENE GLYCOL (P
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enriched with 20 g / l sucrose, 8g
Page 65 and 66: If we compare the two varieties Roc
Page 67 and 68: For Roclas cultivar, the highest nu
Page 69 and 70: RESEARCH ON THE AVERAGE NUMBER OFPO
Page 71 and 72: two weeks before the harvest, were
Page 73 and 74: from minitubersfrom plantlets8.637.
Page 75 and 76: If biological material influences t
Page 77 and 78: BIOTECHNOLOGY OF ORGANIC CULTIVATIO
Page 79 and 80: RESULTS AND DISCUSSIONAccording to
Page 81 and 82: nitrogen sources, barley bran was t
Page 83 and 84: In figure 5 the effects of inoculum
Page 85 and 86: DORMANCY OF SEEDS AND HIS IMPORTANC
Page 87 and 88: Dor. Large samples of grain were ha
Page 89 and 90: Precip.23.06.200524.06.200525.06.20
Page 91 and 92: certificates technologies. In the o
Page 93 and 94: PROBLEMS RELATED TO RECULTIVATION O
Page 95 and 96: Fig. 2 - Density of tests making in
Page 97 and 98: The analysis made of the conditions
Page 99 and 100: The mixtures are intense dynamic bi
Page 101 and 102: Mixed growing of wintering pea and
Page 103 and 104: RHODIOLA ROSEA L. IN VITRO CULTURES
Page 105 and 106: from Rila Mountain National Park of
Page 107 and 108: In vitro seed germination and devel
Page 109 and 110: Along with rhizogenesis, shoot form
Page 111 and 112: 4. Ganzera, M., Yayla, Y., Khan, I.
Page 113 and 114: 1994), while timentin stimulated mo
Page 115: 1 and 2.5 mg/l hyg were not necroti
Page 119 and 120: SECTION II: BIOTECHNOLOGY IN VETERI
Page 121 and 122: The young pigs were fed in accordan
Page 123 and 124: LЕ39,8344,06100,25LЕ29,7944,793,6
Page 125 and 126: During the digestibility test daily
Page 127 and 128: - The use of fodder per unit of liv
Page 129 and 130: Table 1- The observational data for
Page 131 and 132: Having arranged the treatment means
Page 133 and 134: difference between them is 0,78 and
Page 135 and 136: processes that occur during the win
Page 137 and 138: hundreds of fermenting experiences
Page 139 and 140: In the case of spontaneous malolact
Page 141 and 142: With enzymeNo enzymesAstringencyRou
Page 143 and 144: RESEARCHES ON THE BIOTECHNOLOGY POS
Page 145 and 146: from Merlot grapes, with 224 g / l
Page 147 and 148: lasted 12 days, while the variants
Page 149 and 150: case of the produces fermentation.
Page 151 and 152: itself a protective factor against
Page 153 and 154: THE INFLUENCE OF THE CHEMICAL COMPO
Page 155 and 156: Sample witness Thiamine Magnesium s
Page 157 and 158: Both the vitamines (thiamine) and t
Page 159 and 160: This reaction takes place inside th
Page 161 and 162: Sample witness Glycerol 2.5% Glycer
Page 163 and 164: The intensity of the connection can
Page 165 and 166: HIGH NUTRITIVE BIOMASS OF EDIBLE AN
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According to the purpose of this wo
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Experiments were carried out in thr
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Table 5. The sugar and total nitrog
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STUDY OF THE CORRELATIONS BETWEEN G
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The analyzed wheat was characterize
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The lack of a significant relations
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(phenotypically influenced) and glu
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DP700 integrator was used. Compound
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Of the esters were identified:•2-
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Table 4 - Concentration of terpenes
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SECTION IV: INDUSTRIAL AND ENVIRONM
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Table 2. Variation of the compositi
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YoghurtAcerbityFresh butterVerdantF
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1991). This imposes the need of new
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The crop weeds occurrence (mainly w
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formation in the variant fertilized
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gypsum from Sero-Cleaning Installat
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DepthcmTable 1. Water soluble salts
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egulation in mixed filling of ashes
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The aim of this paper is to use the
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RESULTS AND DISCUSSIONPreliminary e
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M1 T1 A B T2 A BM2 T1 A B T2 A BM3
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As one can see in figure 4, the hig
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STUDIES ON IMMOBILIZATION OF CELLUL
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Total immobilizatedprotein (%)80706
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PHYTOREMEDIATION OF LEAD CONTAMINAT
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are placed through holes in the gro
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To highlight phyto-remediation test
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• The leaves have different abili
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include xylanase, endoxylanase, 1,4
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In Taguchi technique, the variation
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REFERENCES1.Amita, R.S., Shah, R.K.
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molecules, such as proteins, DNA, a
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• Quantitative determination of s
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Antioxidant activityDPPH- Free radi
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quantitative determination of the f
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ANTIOXIDANT BIOPRODUCT WITHIMMUNOMO
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evaluations of the specific physica
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TNF-α (pg/ml)100090080070060050040
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THE ENVIRONMENTAL IMPACT OF VITICUL
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hydrocarbon components such as benz
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RESULTS AND DISCUSSIONThe wine indu
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CONCLUSIONIt takes 16 months under
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VANILLIN RELEASE FROM AGAR MICROCAP
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only considering that an amount of
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Fig 4. Surface vanillin (SV) versus
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containing pumpkin extracts (1 ml/l
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Table1 Effect of different types of
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Table 3. Acclimatization of А. mon
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SECTION V: FOOD SAFETYSENSORY ANALY
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Depending on the product analysed,
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Sensory analysis for organic versus
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organic sample), colour (3,85 compa
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Fig. 1.7. Organic (QAF) versus conv
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CONTROL OF MICROBIAL GROWTH AND ENZ
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determined in order to establish th
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3,53log N (N = NTG/g apple slices)2
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a - control; b - apple slices spray
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DETERMINATION OF BIOCHEMICAL MARKER
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Tabel 1. Characteristics of experim
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The maximmal value has been recorde
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Fig 3. Sensors responses for sample
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AN OVERVIEW OF THE METHODS USED TO
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After the sample extraction, the se
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REFERENCES1. Bosset, J. O., Jeangro
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CASE STUDY - METHODS AND FILM PACKA
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Backbone Solution that provides bas
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85 X 55 MM (CREDIT CARD SIZE) OR SM
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the exposing of the entire range of
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REFERENCES1. Agency - March 2002 -
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keep the value of sensory and nutri
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Material exposure to UV radiation w
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Fig. 6. Influence of UV duration on
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Table 3.4. The influence of PEF + U
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SECTION VI: MISCELLANEOUSCOMPUTERIZ
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The computer measures the encasing
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measurements bulletin is typed by t
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5. CONCLUSIONSThe implementation of
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• the knowledge and the informati
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The Agri-Food KMP will be extended,
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REFERENCES1. Abell, A. A. and Oxbro
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quicker translocation of the raw sa
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with X1 [ a,b]such that M ( θ )( )
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as it leads us in the right directi
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