Proceedings Volume 2010 (format .pdf) - SimpBTH

• Quantitative determination of sylimarin, flavonoids, polyphenols and polyphenol-carboxylicacids by spectral technique [21,22].RESULTS AND DISCUSSIONThe obtaining of the flavonoidic vegetal pigments from Agrimonia eupatoria L.and Hypericum perforatum L. speciesThe flavonoidic vegetal pigments (PVA1, PVA2, PVA3 from Agrimoniaeupatoria L. and PVH3, PVH4, PVH4, PVH5 - from Hypericum perforatum L.)were obtained by a succession of technological stages consisting in the first stagein the solid-liquid extraction. Following the extraction procedure, the vegetalmaterial used was removed, and the obtained filtrates were processed by vacuumconcentration until obtaining a residue which, passed through successiveprecipitation with polar and nonpolar solvents, centrifugation, filtering andpurification. The variation of the operational parameters, respectively the finedegree of the plant, the solvent, used the plant/solvent ratio, extraction time andtemperature, resulted in obtaining, non-hygroscopic, fine powdery samples. Thesamples were characterized by spectral techniques UV-VIS (table 2) bychemiluminescence and DPPH methods[23] (Figure 2); the quantitativedetermination of the flavonoids, of the polyphenols, of the polyfenolcarboxilicacids and of specific physical-chemical indicators, were made according to FR X(table 1) [21].Samples Ash % Humidity%Table 1. Physical-chemical characteristicsColourFlavonoids,mass % (asrutin)Polyphenols,mass % (asgallic acid)Polyfenolcarboxilicacids, mass % (ascaffeic acid)PVA1 2.19 2.81 Yellowbrown3.54 4.501 2.573PVA2 2.54 2.67 Yellow 3.02 3.735 1.987PVA3 2.31 2.15 Yellowbrown3.21 2.948 1.712PVH4 2.58 3.07 Red-brown 3.49 3.917 2.998PVH5 2.83 3.11 Greenbrown4.38 4.341 3.112PVH6 2.21 2.64 brown 2.41 3.136 1.821PVH7 2.79 3.28 Red-brown 3.27 5.786 3.124UV-VIS characteristics of the samplesThe samples in ethanolic solvent have similar electronic spectra which those comefrom structures with an extended conjugation (table 2 and figure 1).233