Proceedings Volume 2010 (format .pdf) - SimpBTH

Along with rhizogenesis, shoot formation continued bringing to regeneration ofplants with different propagation rate depending on media composition for seedlingdevelopment (G1-G4) and for rooting of shoots (V1-V8). The highestmultiplication coefficient was recorded for Scheme I followed by III rd , II nd and IV thschemes.Fig 2. Rhodiola rosea in vitro rooting and new shoot formation onV8 medium.Adaptation. Adaptation of obtained in vitro plants into their natural environmentis one of the most delicate and difficult stages in elaborating micropropagationprocedures. Usually regenerants formed well-developed root system. However,they quickly loose their turgor after transfer to soil. Their leaves withered anddried. These plants underwent stress due to the changes in humidity and culturemedium. Our results showed that high air humidity of 80 % to 90 % during the first5-10 days of acclimatization is very important for further plant survival (Table 5).The type of rooting substrates (soil, peat and perlite) has dramatic impact onplantlets development. Used in a single manner each of the substrates is ineffective(variants 1-4, Table 5). Combination of soil and perlite or peat brought to survivalof 10-22 %. Best combination is soil, peat and perlite in ratio of 2:1:1 (variant 9).The same rooting medium is much more efficient when air humidity is very high –from 80 % to 90 % (Fig. 3).Fig 3. Plant adaptation on rooting substrates (variant10).In this environment, survival of plantlets is nearly 60 % and their furtherdevelopment in wild nature in the mountains was possible.109