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Table 3. Chemiluminescence characteristicsSample code k (s -1 ) v i (s -1 ) AA % [CL]PVA 1 0.102 120.51 92.40PVA 2 0.095 291.48 87.10PVA 3 0.090 104.21 89.70PVH 4 0.086 145.24 90.15PVH 5 0.100 118.84 94.02PVH 6 0.116 183.21 80.01PVH 7 0.094 291.74 78.91Quercetine 0.101 128.83 92.80Kaemferol 0.105 124.80 92.49Rutine 0.093 291.40 77.40Apigenin 0.098 289.20 82.62Chlorogenic acid 0.096 100.00 87.20Caffeic acid 0.070 318.00 82.90Galic acid 0.112 166.46 85.70CL [a.u.]6 50 06 00 05 50 05 00 04 50 04 00 03 50 03 00 02 50 02 00 01 50 01 00 050 0P V A 2P V A 1P V A 300 5 0 1 00 1 5 0 2 00 25 0 3 00T im e [s]Fig 2. CL evolution in time of the samples PVA1-PVA3Antioxidant activity and the rate constants of the reaction (table 3)emphasize high protective capacity of the extracted components. The antioxidantactivity of the flavonoidic vegetal pigments is attributed to the active principles,especially to the flavonoids content.The qualitative and quantitative content of the phenolic and flavonoidicconstituents in the samples, confirmed by spectral analysis and chemiluminescencedeterminations, explain the high value of the antioxidant activity, especially for thesample PVH 5 (94.02%) [25].CONCLUSIONSThe extractive process conducted to the obtaining of seven flavonoidic vegetalpigments from Agrimonia eupatoria L. and Hypericum perforatum L. Spectralinvestigations (UV-VIS) on the flavonoidic vegetal pigments emphasized thepresence of some phenolic and flavonoidic structures, also confirmed by236
quantitative determination of the flavonoids, polyphenols and polyphenolcarboxilicacids.The results of the free radical-scavenger activity was determined by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay revealed that the samples exhibit highantioxidant activity, being in accordance with the values provided bychemiluminescence in vitro tests, and suggesting thus their potential application forprophylaxy and therapy of various free radical related diseases.Experimental dataemphasized the structural complexity and antioxidative capacity of the flavonoidicvegetal pigments.A correlation between antioxidant activity and flavonoidic content wasobserved for all the flavonoidic vegetal pigments. The results obtainedrecommending the flavonoidic vegetal pigments from Agrimonia eupatoria L. andHypericum perforatum L. species for applications in pharmaceutical industry,cosmetic industry and food additives.REFERENCES1. Middleton E, Jr., Kandaswami C. and Theoharides TC. (2000): The effects of plant flavonoids onmammalian cells: implications for inflammation, heart disease, and cancer. Pharmacology Review,Vol.52:673.2.Pietta PG. (2000): Flavonoids as antioxidants. Journal of Natural Products Vol. 63: 1035.3.Ben-Yoseph, O., Boxer, P. A., & Ross, B. D. (1996). Assessment of therole of the glutathione andpentose phosphate pathways in theprotection of primary cerebrocortical cultures from oxidativestress. Journal of Neurochemistry, Vol. 66: 2329–2337.4.Fridovich, I. (1997). Superoxide anion radical, superoxide dismutases, and related matters. Journalof Biological Chemistry, Vol. 272, 18: 515-517.5.Halliwell, B. (1996). Oxidative stress, nutrition and health. Experimental strategies for optimizationof nutritional antioxidant intake in humans. Free Radical Research, Vol. 25:, 57–74.6.Halliwell, B. (1999). Establishing the significance and optimal intake of dietary antioxidants: Thebiomarker concept. Nutrition Reviews, Vol. 57:, 104–113.7.Behl, C., & Mosmann, B. (2002). Antioxidant neuroprotection in Alzheimer’s disease as preventiveand therapeutic approach. FreeRadical Biology and Medicine, Vol. 33:, 182–191.8.Foy, C. J., Passmore, A. P., Vahidassr, M. D., Young, I. S., & Lawson,J. T. (1999). Plasma chainbreakingantioxidants in Alzheimer’s disease, vascular dementia, and Parkinson’s disease. QJMJournal of the Association of Physicians, Vol. 92: 39–45.9.Gardner, P. T., White, T. A. C., McPhail, D. B., & Duthie, G. G. (2000). The relative contributionsof vitamin C, carotenoids and phenolics to the antioxidant potential of fruit juices. Food Chemistry,Vol. 68:, 471–474.10.Youdim, K. A., Dobbie, M. S., Kuhnle, G., Proteggente, A. R.,Abbott, N. J., & Rice-Evans, C.(2003). Interaction between flavonoids and the blood–brain barrier: In vitro studies. Journal ofNeurochemistry, Vol. 85: 180–192.11.Zheng, W., & Wang, S. Y. (2001). Antioxidant activity and phenolic compounds in selected herbs.Journal of Agricultural and FoodChemistry, Vol.49 : 5165–5170.12. H. Sies, E .Cadenas, (1985), Oxidative stress, Vol.311: 617-631.13. A.S. Pannala, R. Razaq, B. Halliwell, S. Singh, C. Rice-Evans, (1998) Free Rad.Biol. Med.,Vol.24: 594-606.237
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UNIVERSITY OF AGRONOMICAL SCIENCES
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SCIENTIFIC COMMITTEEProf. Dr. Petru
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CONTENTSECTION I: AGRICULTURAL BIOT
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SECTION IV: INDUSTRIAL AND ENVIRONM
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Proceeding of the 3 rd Internationa
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All the biological material (positi
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At harvesting, symptoms could be in
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Necrotic Disease (PTRND) induced by
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Eppendorfer, W., H., Eggum, B.,O.,
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Now Lamium genus from Lamiaceae fam
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extracts and rutin, hyperoside, chl
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Figure 3. Scavenging activity on DP
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CO-TRANSFORMATION OF POTATO (SOLANU
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transferred OCI and OCII genes were
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correspond to co-transformation fre
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EXPERIMENTS ON LASER RADIATION INFL
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formations. The average percentage
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Legend: Variants: V1 (5 minutes of
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We thank our partners at 4R OPTICS
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Regarding superior plants they are
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Fig. 2. The mechanism of the abscis
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In figure 3 it is presented the bio
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In the same time it has been proved
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egenerative lines of alfalfa, Wan e
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demonstrated direct regeneration fr
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(Knoll et al. 1997, Zhang and Zeeva
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the mean ± standard error. Six sam
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IMPACT OF PHOTOPERIOD ON SPINACH RE
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etween SD and LD response of the sa
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GA 3 plays an important role in spi
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INFLUENCE OF POLYETHYLENE GLYCOL (P
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enriched with 20 g / l sucrose, 8g
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If we compare the two varieties Roc
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For Roclas cultivar, the highest nu
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RESEARCH ON THE AVERAGE NUMBER OFPO
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two weeks before the harvest, were
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from minitubersfrom plantlets8.637.
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If biological material influences t
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BIOTECHNOLOGY OF ORGANIC CULTIVATIO
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RESULTS AND DISCUSSIONAccording to
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nitrogen sources, barley bran was t
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In figure 5 the effects of inoculum
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DORMANCY OF SEEDS AND HIS IMPORTANC
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Dor. Large samples of grain were ha
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Precip.23.06.200524.06.200525.06.20
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certificates technologies. In the o
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PROBLEMS RELATED TO RECULTIVATION O
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Fig. 2 - Density of tests making in
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The analysis made of the conditions
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The mixtures are intense dynamic bi
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Mixed growing of wintering pea and
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RHODIOLA ROSEA L. IN VITRO CULTURES
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from Rila Mountain National Park of
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In vitro seed germination and devel
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Along with rhizogenesis, shoot form
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4. Ganzera, M., Yayla, Y., Khan, I.
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1994), while timentin stimulated mo
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1 and 2.5 mg/l hyg were not necroti
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Nevertheless, we suggest stepwise i
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SECTION II: BIOTECHNOLOGY IN VETERI
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The young pigs were fed in accordan
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LЕ39,8344,06100,25LЕ29,7944,793,6
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During the digestibility test daily
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- The use of fodder per unit of liv
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Table 1- The observational data for
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Having arranged the treatment means
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difference between them is 0,78 and
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processes that occur during the win
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hundreds of fermenting experiences
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In the case of spontaneous malolact
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With enzymeNo enzymesAstringencyRou
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RESEARCHES ON THE BIOTECHNOLOGY POS
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from Merlot grapes, with 224 g / l
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lasted 12 days, while the variants
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case of the produces fermentation.
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itself a protective factor against
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THE INFLUENCE OF THE CHEMICAL COMPO
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Sample witness Thiamine Magnesium s
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Both the vitamines (thiamine) and t
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This reaction takes place inside th
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Sample witness Glycerol 2.5% Glycer
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The intensity of the connection can
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HIGH NUTRITIVE BIOMASS OF EDIBLE AN
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According to the purpose of this wo
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Experiments were carried out in thr
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Table 5. The sugar and total nitrog
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STUDY OF THE CORRELATIONS BETWEEN G
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The analyzed wheat was characterize
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The lack of a significant relations
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(phenotypically influenced) and glu
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DP700 integrator was used. Compound
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Of the esters were identified:•2-
Page 185 and 186: Table 4 - Concentration of terpenes
Page 187 and 188: SECTION IV: INDUSTRIAL AND ENVIRONM
Page 189 and 190: Table 2. Variation of the compositi
Page 191 and 192: YoghurtAcerbityFresh butterVerdantF
Page 193 and 194: 1991). This imposes the need of new
Page 195 and 196: The crop weeds occurrence (mainly w
Page 197 and 198: formation in the variant fertilized
Page 199 and 200: gypsum from Sero-Cleaning Installat
Page 201 and 202: DepthcmTable 1. Water soluble salts
Page 203 and 204: egulation in mixed filling of ashes
Page 205 and 206: The aim of this paper is to use the
Page 207 and 208: RESULTS AND DISCUSSIONPreliminary e
Page 209 and 210: M1 T1 A B T2 A BM2 T1 A B T2 A BM3
Page 211 and 212: As one can see in figure 4, the hig
Page 213 and 214: STUDIES ON IMMOBILIZATION OF CELLUL
Page 215 and 216: Total immobilizatedprotein (%)80706
Page 217 and 218: PHYTOREMEDIATION OF LEAD CONTAMINAT
Page 219 and 220: are placed through holes in the gro
Page 221 and 222: To highlight phyto-remediation test
Page 223 and 224: • The leaves have different abili
Page 225 and 226: include xylanase, endoxylanase, 1,4
Page 227 and 228: In Taguchi technique, the variation
Page 229 and 230: REFERENCES1.Amita, R.S., Shah, R.K.
Page 231 and 232: molecules, such as proteins, DNA, a
Page 233 and 234: • Quantitative determination of s
Page 235: Antioxidant activityDPPH- Free radi
Page 239 and 240: ANTIOXIDANT BIOPRODUCT WITHIMMUNOMO
Page 241 and 242: evaluations of the specific physica
Page 243 and 244: TNF-α (pg/ml)100090080070060050040
Page 245 and 246: THE ENVIRONMENTAL IMPACT OF VITICUL
Page 247 and 248: hydrocarbon components such as benz
Page 249 and 250: RESULTS AND DISCUSSIONThe wine indu
Page 251 and 252: CONCLUSIONIt takes 16 months under
Page 253 and 254: VANILLIN RELEASE FROM AGAR MICROCAP
Page 255 and 256: only considering that an amount of
Page 257 and 258: Fig 4. Surface vanillin (SV) versus
Page 259 and 260: containing pumpkin extracts (1 ml/l
Page 261 and 262: Table1 Effect of different types of
Page 263 and 264: Table 3. Acclimatization of А. mon
Page 265 and 266: SECTION V: FOOD SAFETYSENSORY ANALY
Page 267 and 268: Depending on the product analysed,
Page 269 and 270: Sensory analysis for organic versus
Page 271 and 272: organic sample), colour (3,85 compa
Page 273 and 274: Fig. 1.7. Organic (QAF) versus conv
Page 275 and 276: CONTROL OF MICROBIAL GROWTH AND ENZ
Page 277 and 278: determined in order to establish th
Page 279 and 280: 3,53log N (N = NTG/g apple slices)2
Page 281 and 282: a - control; b - apple slices spray
Page 283 and 284: DETERMINATION OF BIOCHEMICAL MARKER
Page 285 and 286: Tabel 1. Characteristics of experim
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The maximmal value has been recorde
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Fig 3. Sensors responses for sample
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AN OVERVIEW OF THE METHODS USED TO
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After the sample extraction, the se
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REFERENCES1. Bosset, J. O., Jeangro
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CASE STUDY - METHODS AND FILM PACKA
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Backbone Solution that provides bas
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85 X 55 MM (CREDIT CARD SIZE) OR SM
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the exposing of the entire range of
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REFERENCES1. Agency - March 2002 -
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keep the value of sensory and nutri
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Material exposure to UV radiation w
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Fig. 6. Influence of UV duration on
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Table 3.4. The influence of PEF + U
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SECTION VI: MISCELLANEOUSCOMPUTERIZ
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The computer measures the encasing
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measurements bulletin is typed by t
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5. CONCLUSIONSThe implementation of
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• the knowledge and the informati
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The Agri-Food KMP will be extended,
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REFERENCES1. Abell, A. A. and Oxbro
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quicker translocation of the raw sa
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with X1 [ a,b]such that M ( θ )( )
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as it leads us in the right directi
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