Proceedings Volume 2010 (format .pdf) - SimpBTH

INFLUENCE OF POLYETHYLENE GLYCOL (PEG)CONCENTRATIONS ON WATER STRESS INDUCINGANDREEA NISTOR 1 , GH. CÂMPEANU 2 , N. ATANASIU 2NICOLETA CHIRU 1 , DIANA KARACSONYI 11 INCDCSZ, Braşov, tican_andreea@yahoo.com2 USAMV , BucureştiKeywords: plantlets, concentration, growth, height, leavesABSTRACTDrought causes changes in growth of plants. Tissue culture is useful in studying themechanism of tolerance to water stress “in vitro” conditions. Culture technique “in vitro” reducedvariation due to medium (the nutria medium, controlled conditions). Polyethylene glycol is used insimulating water stress for plants and as such, and the influence of PEG was studied to NIRDPSBBrasov. It had been experienced over the plantlets “in vitro”, on Romanian cultivars. Influence ofmodified media supplemented with polyethylene glycol on growth in height of plantlets and number ofleaves/plantlets obtained “in vitro” was different in the two cultivars tested.INTRODUCTIONDrought is one of the most common environmental stresses affecting plantgrowth and productivity (Boyer, 1982). Plant cell and tissue culture has been anuseful tool to study stress tolerance mechanisms under in vitro conditions (Bajji etal., 2000). Water deficit is a common stress in potato production, which leads todecrease in tuber quality and yield. Because of potato susceptibility to drought(Hassanpanah et al, 2008), preparing sufficient water is very important forincreasing potato quality and quantity.It is very important to study about tolerance of different potato cultivarsagainst water deficit stress. Water deficit decreased number of leaves, plant waterpotential (Frensch, 1997), leaf area, stem height, ground coverage, tuber number,growth and yield.In this study, our aim is to determine the potential reactions of plants todrought “in vitro” by inducing water stress using polyethylene glycol (PEG).MATERIAL AND METHODIn Plant Tissue Culture Laboratory of NIRDPSB Brasov, in 2009, wasfitted the experience that followed the influence of polyethylene glycol PEG's onthe plantlets, experience bifactorial, with 10 options, divided into three repetitions.To achieve the experience, have been studied experimental variantspresented in next table:61