Proceedings Volume 2010 (format .pdf) - SimpBTH

from seeds germinated on different media, multiplied on Z-5 medium and rootedon various nutrient media.Rooting. Plant regenerants 2.5 to 3.5 cm in size were transferred for rooting oneight different nutrient media containing GA 3 in combination of three auxins.Rhizogenesis of more than 58 % was observed on the all variants except of V2forming callus. This medium contained NAA in contrast to the other mediacontaining IBA or IAA. The process of rhizogenesis induction, root length andformation of new shoots from an explant was recorded for the different variants ofculture media.The most efficient rhizogenesis concerning percentage of root induction (higherthan 85 %) and root length (longer than 2 cm) was observed when the first scheme(seed germination on G1 medium, seedling explants development on Z-5 mediumand induction of rhizogenesis on all rooting media (V1, V3-V8 variants) (Table 4).Table 4. Effect of culture media composition on the processes of rooting and propagation.Rooting108Scheme I Scheme II Scheme III Scheme IVSeedling growth on G1medium, explantdevelopment on Z-5,transfer of shoots onrooting media:Seedling growth on G2medium, explantdevelopment on Z-5,transfer of shoots onrooting media:Seedling growth on G3medium, explantdevelopment on Z-5,transfer of shoots onrooting media:Seedling growth on G4medium, explantdevelopment on Z-5,transfer of shoots onrooting media:media (1) (2) (3) (1) (2) (3) (1) (2) (3) (1) (2) (3)V1 92 2.0±0.034.0±0.1376 2.2±0.063.0±0.1190 1.8±0.104.4±0.0678 1.5±0.122.6±0.13V2 CalliV3 85.7 1.63 2.26 43.6 2.07 1.27 86.7 1.14 1.18 65 1.08 2.65V4 96 2.5±0.096.0±0.1193 2.5±0.093.4±0.1392 1.6±0.104.5±0.0988 1.4±0.076.5±0.17V5 85 1.6±0.075.5±0.1367 2.0±0.032.5±0.1278 1.5±0.084.0±0.0372 1.8±0.055.0±0.19V6 87 1.5±0.095.0±0.1072 1.2±0.094.0±0.0576 1.5± 4.5±0.0864 1.8±0.145.0±0.01V7 92 1.9±0.104.5±0.0395 1.0±0.034.8±0.1448 1.8±0.162.5±0.0842 1.2±0.093.0±0.02V8 96 2.3±0.056.0±0.1088 2.0±0.035.5±0.0698 2.4±0.086.5±0.0497 2.2±0.127.4±0.09Legends: (1) Rooting [%]; (2) Root Length [cm]; (3) Propagation rateHigher rooting efficiency of more than 85 % in four out of seven cases andrelatively long roots was recorded for Scheme III where seed germination was onG3 medium and rooting of shoots was on V1, V4 and V8 media. Schemes II andIV were less efficient. Concerning the best rooting media – V8 and V4 were rankedat the first two places (Fig. 2). The both media contained IBA 2 mg/l, IAA 0.2 mg/land GA 3 , however, in different concentration – 1 mg/l for V4 and 0.4 mg/l for V8medium.