Proceedings Volume 2010 (format .pdf) - SimpBTH

At harvesting, Potato Tuber Ring Necrotic Disease symptoms could beindentified on the tubers from all the other varieties, excepting the cv. Sante,Christian and Ostara. Concerning the other varieties, the appearence and evolutionof symptoms on tubers is going on imediatly after harvesting.After 3 months from harvesting the inoculated material, the frequency oftubers with symptoms was between 6.6-22.2% for varieties Dacia, Agata, Romano,Roclas, Ostara and for cv. Tampa, Koretta, Hermes this percentage was very high(60.0-95.8%).The samples with significantly higher protein content (cv. Sante andChristian) were resistent to PVY NTN inoculation. In this case, after 3 months fromharvest, the stored tubers didn’t have visible tuber necrotic ringspot diseasesymptoms.Acknowledgements We gratefully acknowledge Dr. Enache V., dr. Necula I. and dr. RomanMonica from Sanitary Veterinary and Food Safety Agency Brasov, for their substantialcontributions to this research effort.REFERENCESBădărău, C., L., Chiru, S.,C., Cojocaru, N., Ianosi, M., Chiru, N. (2010): Studies regarding theimprovement of methods used for viruses identification in potato seed indexation. In: Potatoagrophysiology. Proceedings of the International Symposium on Agronomy and Physiology ofPotato, Nevsehir, Turkey: 332-340Bădărău, C.,L., Mărculescu, A., Cojocaru, N., Rusu, S., N., Ianoşi, M. (2010): Studies regarding theimprovement of methods used for the potato’s viruses identification- Bulletin Issue ofInternational Conference on New Research in Food and Tourism Bioatlas , Brasov, 28-30 May2010, Journal of EcoAgroTurism, Transilvania University of Brasov Publisher: 83-91Bădărău, C.,L., Cojocaru, N., Rusu, S.,N., Ianoşi, M., Petrusca, K. (2009):The effect of samplesincubation on detection of PLRV and the influence of several extraction buffer’s additives on thedetection of potato viruses Y, A, X and S by ELISA technique. In Proceeding of the 2ndInternational Symposium “ New Researches in Biotechnology, Series F (Special volume),Biotechnology, 2009, Bucharest : 9-17Beczner, J,, Horvath, J, Romhanyi, L., Forster, H. (1984): Studies on the etiology of tuber necroticringspot disease in potato. Potato Research, 27:339-352Beemster, A., B., R., de Bokx, J., A. (1987): Survey of properties and symptoms. In Viruses of potatoand seed potato production, eds. J.A.de Bokx and J.P.H. van der Want, Wageningen, TheNetherlands RUDOC: 284-290;Clark, M.,F., Adam, A.,N., (1977): Characteristics of microplate method of enzyme linkedimmunosorbent assay for the detection of plant viruses. J. Gen Virol., 34: 475-483.Cojocaru, N., Bădărău, C.,L., Doloiu, M. (2009): Potato virus Y (PVY) purification and achievementof antisera for ELISA identification of infected plants. In Proceeding of the 2nd InternationalSymposium “ New Researches in Biotechnology”, Series F (Special volume), Biotechnology,2009, Bucharest : 18-25Davis, J.,A. and Radcliff ,E.,B.,Schrage, W., Rgsdale, D.,W. (2008): Vector and virus IPM for seedpotato production.In Insect pest management: Concepts, tactics, strategies and case studies, eds.Radcliffe E.B., Huchison W.D., Cancelado R.E., Cambridge, UK, Cambridge UniversityPress:366-37716