Proceedings Volume 2010 (format .pdf) - SimpBTH

Now Lamium genus from Lamiaceae family is less studied and turned toaccount (valorized), comparing to other members of this family.The genus Lamium (Lamiaceae) comprises about 40 species distributed in Europe,Asia and Africa. Some Lamium species have been used in traditional and folkmedicine. The most popular is L. album. [4]Besides this species, in Romania also grow L. maculatum, L. bifidumCirillo ssp. balcanicum Vel, L. galeobdolon L., L. amplexicaule, L. bithynicumBenth., L. bifidum Cirillo ssp. balcanicum and L. purpureum. Lamium purpureumis less studied, but this species shows a special pharmacological potential due to thefamily that belong to.This study intents to show the antioxidant potential of 4 extracts fromnative Lamium species -Lamium album and Lamium purpureum-, emphasizing thecorrelation between chemical composition and biological effect.2. MATERIALS AND METHODSChemicals: Folin-Ciocalteu's phenol reagent, 2.2-Di(4-tert-octyphenyl)-1-picrylhydrazyl (DPPH), caffeic acid, chlorogenic acid, gallic acid, rutin,hyperoside were purchased from Sigma Chemical Co. Dried vegetal material waspurchase from a local drugstore (Lamium album, Lamium purpureum)Methanol, ethanol and acetone were used as dissolving/extracting solvents.Ethyl acetate, formic acid, and distilled water (p.a) as TLC developants.Preparation of plant extracts – Extracts were obtained by:- succesive (repetitive) methanolic extractionLp1 Lamium purpureum (methanol 80%)La1 Lamium album (methanol 30%)and- repetitive acetonic extractionLp2 Lamium purpureum (acetone 50%)La2 Lamium album (acetone 50%).vegetal material/ solvent rate -1/10 m/v at the boiling temperature of the solvent for2 hours first time and 1 hour second time, under continuous stirring. Afterfiltration, the reunited methanolic/ acetonic solution was concentrated underreduced pressure (72-74 mm Hg) and 50°C to a vegetal material/methanolic(acetonic) extract rate - 1/1.5 (m/v). The insoluble substances were removed byalcohool precipitation and after 24 hours repaos was centrifuged at 3000 rpm for30 minutes. The resulted solution was concentrated under reduced pressure (72-74mm Hg) and 50°C to a vegetal material/methanolic (acetonic) extract rate - 1/1(m/v) and spray-dried.Determmination of flavones: Qualitative analyses for flavones were carriedout usig TLC methods [5]. Quantitative analysis for flavones were made usig a19