Proceedings Volume 2010 (format .pdf) - SimpBTH

Table 5. Survival of Rhodiola rosea plants regenerated in vitro after transfer to rootingsubstrates.Variant Rooting substrates (components andproportions)Air humidity[%]Survival[%]1 Soil 60 - 70 42 Soil 80 - 90 23 Peat 80 - 90 24 Perlite 60 - 70 Roots get rot5 Soil + perlite (3:1) 60 - 70 106 Soil + perlite (3:1) 80 - 90 127 Soil + peat (3:1) 60 - 70 108 Soil + peat (3:1) 80 - 90 229 Soil + peat + perlite (2:1:1) 60 - 70 3510 Soil + peat + perlite (2:1:1) 80 - 90 57CONCLUSIONSGolden root seed germination started on the 7 th day of cultivation and lasteduntil the 40 th day reaching from 37.5 % to 97.0 % depending on mediacomposition. Highest level of proliferation on Z-5 medium was observed in May-June, when the number of shoots per stem explant was 6.78. During cold seasonsmultiplication was relatively lower - 2.11 shoots per explant. The most efficientrhizogenesis concerning percentage of root induction (higher than 85 %) and rootlength (longer than 2 cm) was observed on the first scheme (seed germination onG1 medium, seedling explants development on Z-5 medium and induction ofrhizogenesisi on all rooting media (V1, V3-V8 variants). Best combination forplant adaptation was soil, peat and perlite in ratio of 2:1:1 and very high airhumidity (80 % to 90 %). In this environment, survival of plantlets is nearly 60 %and their further development in wild nature in the mountains was possible.ACKNOWLEDGEMENTSConsultation of Dr. L. Evstatieva from the Institute of Botany, BulgarianAcademy of Sciences is greatly appreciated. Research was supported by NationalScience Fund – Project CC MU 1201 (leader K. Tasheva) and ProjectBG051PO001/07/3.3-02/25/08REFERENCES1. Brown, R. P., Gerbarg, P. L., Ramazanov, Z. (2002): Rhodiola rosea: A Phytomedicinaloverview. Herbal Gram. Vol. 56, 40-52.2. Dimitrov, B., Tasheva, K., Zagorska, N., Evstatieva, L. (2003): In vitro cultivation of Rhodiolarosea L. Genetics and Breeding. Vol. 32, 1-2: 3-6.3. Farnsworth, N.R., Soejarto, D.D. (1991): Global importance of medicinal plants. TheConservation of Medicinal Plants. In: Akerele, O., Heywood, V., Synge, H. (Eds.), CambridgeUniversity Press, Cambridge. 25–52.110