Living Image 3.1

Living Image ® Software User’s Manual
6
Image Math
Using Image Math to Create a New Image . . . . . . . . . . . . . . . . . . . . . . . . . . 98
Subtracting Tissue Autofluorescence . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101
Overlaying Multiple Images . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 104
The Living Image ® software provides tools that enable you to mathematically combine
two images to create a new image. The primary use of image math is to subtract tissue
autofluorescence background from signal.
LIving Image Tool Use This Tool To... See Page
Image Math Mathematically combine (add, multiply, subtract, or divide)
two user-specified images.
98
Image Math Remove autofluorescence from a fluorescent image. 101
Image Overlay Coregister multiple fluorescent or luminescent images on the
same photographic image to view multiple reporters in a
single image.
104
To perform image math, open an image sequence (see page 37) or a group of images
(see Creating an Image Sequence from Individual Images, page 43).
97