Living Image 3.1

11. 3D Reconstruction of Sources
Viewing Photon
Density
146
Table 11.3 3D reconstruction results
Item Description
Tissue Properties The user-specified tissue in which the
sources should be located.
Source Spectrum The emission spectrum of the type of bioluminescent
source.
Photon Density Maps
Simulated The photon density computed from DLIT/FLIT source
solutions which best fit the measured photon density (see
page 147).
Measured The photon density determined from the image
measurements of surface radiance.
Wavelength The wavelength of the photon density map in the active
image.
Source Image The image number of the transillumination source image.
Photon Density Maps
Save Results
Click to open the Photon Density Maps window.
Name The default name for the active DLIT or FLIT results.
Delete Click to delete the selected DLIT or FLIT results.
Load Click to load the selected DLIT or FLIT results.
Save Click to save the active DLIT or FLIT results.
Overwrite If you reanalyze saved results, click to save the new results
and overwrite the previous results.
Photon density is the steady state measure of the number of photons in a cubic
millimeter. Light sources inside the tissue contribute to photon density in other portions
of the tissue. The DLIT or FLIT algorithm first converts the luminescent image of
surface radiance to photon density just below the animal surface because this is what
can be observed. Then the DLIT or FLIT algorithm solves for point source locations
inside the tissue which would produce the observed photon density near the surface.
To check the quality of the DLIT or FLIT construction, it is useful to compare the
measured and simulated photon density plots. The photon density is closely related to
the measured radiance.