Living Image 3.1

G. Planar Spectral Imaging
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To estimate the cell count, divide the absolute photon flux by the flux per cell. This is best
determined by making independent in vitro measurements of the cell line used in the
experiment.
The Plot of Linear Fit Results is weighted by the uncertainty of the measured images and
takes into account the uncertainty in the determination of the optical properties. The
precision of the method is largely determined by the known precision of the optical
properties. In most cases, the relative uncertainty in the depth determination is equal to the
relative uncertainty in the optical properties.
An analysis of the dorsal and ventral views of the mouse left lung in Figure G.5 results in
total flux values that are very similar. The measured depth values are also close, indicating
that the cells are distributed about the same distance from the front and back of the animal.
Figure G.5 Planar spectral analysis results
Top: Dorsal view of the left lung, bottom: ventral view of the left lung