Living Image 3.1

Living Image ® Software User’s Manual
Table 3.1 Image window
Item Description
Units Select the measurement units for the image display (counts or photons) from
this drop-down list
Display Select the image type (for example, overlay) that you want to display from this
drop-down list. For more details on the different types of image displays, see
Figure 4.5, page 47.
Info Click to display or hide the image label information.
Opens a dialog box that enables you to export the active view as a graphic file.
Color bar Shows the minimum and maximum pixel intensities in the image as well as the
color bar. Pixels less than the color bar minimum or greater than the color bar
maximum are not displayed in the image.
Image label Information about the image that the software automatically records and userspecified
information entered in the Edit Image Label dialog box.
3.5 Acquiring a Single Fluorescence Image With Epi-Illumination
Epi-illumination uses a excitation light source located above the stage. For more details
on fluorescence imaging, see page 169.
1. Start the Living Image software (double-click the icon on the desktop).
2. Initialize the IVIS System and confirm or wait for the CCD temperature to lock. (For
more details, see page 11.)
3. Put a check mark next to Fluorescent.
4. Confirm that the Fluorescent Lamp Level is set to the appropriate level (High or
Low).
5. Select the Auto exposure time (click the arrow). Alternately, manually set the
exposure, binning, and F/Stop. (For more details on the control panel settings, see
page 177.)
6. Set the FOV: To adjust the field of view (FOV), make a selection from the Field of
View drop-down list. For more details on FOV, see page 160.
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