Living Image 3.1

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I. Menu Commands, Tool Bar, & Shortcuts Table I.1 Menu bar commands and toolbar buttons Menu Bar Command Toolbar Button Edit ➞ Copy Copies the active image window to the system clipboard. Edit ➞ Image Labels Opens the Edit Image Labels dialog box that enables you to edit the label set information for the active data. Edit ➞ Preferences Opens the Preferences box. View ➞ Tool Bar Choose this option to display the toolbar. View ➞ Status Bar Choose this option to display the status bar at the bottom of the main window. View ➞ Activity Window Displays the Activity window at the bottom of the main application window. The Activity window shows a log of the system activity. View ➞ Tool Palette Choose this option to display the tool palette. View ➞ Activity Window Choose this option to display the activity bar at the bottom of the main window. The activity bar lists a history of the recent software activities. View ➞ Image Information Displays the Image Information box that shows the label set and image acquisition information for the active data. View ➞ ROI Properties Displays the ROI Properties dialog box. View ➞ ROI Measurements Displays the ROI Measurements table. View ➞ Image Layout Window Opens the Image Layout window that enables you to paste an image of the active data in the window. View ➞ Acquisition Control Panel Displays the control panel. Tools ➞ Image Math for... Opens the Image Math window for the active data. Tools ➞ Image Overlay for... Opens the Image Overlay window for the active data. Tools ➞ Colorize xx_SEQ Opens the Colorized View tab for the active sequence. Note: This menu item is only available if “Show Advanced Options” is selected in the Preferences. Tools ➞ Transillumination Overview Acquisition ➞ Background ➞ Replace Acquisition ➞ Background ➞ View Available Dark Charge Acquisition ➞ Background ➞ Clear Available Dark Charge Acquisition ➞ Background ➞ Measure Acquisition ➞ Fluorescent Background ➞ Add or Replace Fluorescent Background Acquisition ➞ Fluorescent Background ➞ Measure Fluorescent Background Acquisition ➞ Fluorescent Background ➞ Add or Replace Fluorescent Background 244 Description Combines the images of a FLIT sequence into a single image (intensities are summed) that can be analyzed using tools in the tool palette. Opens a dialog box that enables you to select an instrument luminescent background. This background measurement is subtracted from luminescent images. Opens a dialog box that enables you to view the dark charge measurements for the system. Opens a dialog box that enables you to remove the dark charge measurements from the system. Opens a dialog box that enables you to acquire a dark charge measurement. Opens a dialog box that enables you to select an instrument fluorescent background measurement for the active image data. If the Sub Fluor Bkg option is chosen in the control panel, the background measurement is subtracted from the image data. Starts a measurement of the instrument fluorescent background. Opens a dialog box that enables you to select a fluorescent background measurement.
Living Image ® Software User’s Manual Table I.1 Menu bar commands and toolbar buttons Menu Bar Command Toolbar Button Acquisition ➞ Fluorescent Background ➞ Clear Available Fluorescent Background Acquisition ➞ Fluorescent Background ➞ View Available Fluorescent Background Opens a dialog box that enables you to remove the fluorescent background measurements from the system. Opens a dialog box that displays the fluorescent background measurements for the system. If a fluorescent background is selected, the Sub Fluor Bkg option appears in the control panel. Choose the Sub Fluor Bkg option to subtract the user-specified background measurement from the image data. Window ➞ Close Closes the active image window. Window ➞ Close All Closes all image windows. Window ➞ Cascade Organizes the open image windows in a cascade arrangement (see page 48). Window ➞ Tile Organizes the open image windows in a tiled arrangement (see page 49). Window ➞ 1. xx A list of the open data. Select the data of interest to it the active window and Window ➞ 2. xx Window ➞ etc. display it on top of all other open windows. Window ➞ Other Windows ➞ Browser Window Description If the Living Image browser is open, makes it the active window and displays it on top of all other open windows. Help ➞ User Guide Displays the online help. Help ➞ About Living Image Displays the online help index. Click this button, then click an item in the user interface to display information about the item. Table I.2 Keyboard shortcuts Keys Shortcut Description Ctrl + B Opens the Living Image browser. Ctrl + C Copies the active image to the system clipboard. Ctrl + D Arranges open windows in a cascade. Ctrl + O Displays a dialog box that enables you to open data. Ctrl + P Open the Print dialog box. Ctrl + S Saves the active file or window. Ctrl + T Tiles the open windows. Ctrl + W Closes the active window. Shift + F1 Changes the mouse pointer to the “What’s This” tool . Click this button, then click an item in the user-interface to display information about the item. 245
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Living Image 3.1

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