Living Image 3.1

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8. Spectral Unmixing 8.3 Spectral Unmixing Parameters 118 Table 8.1 Tool palette, spectral unmixing tools Item Description SOL A spectrum generated by the spectral unmixing algorithm. ROI A spectrum calculated for a user-selected ROI. LIB A user-selected library spectrum. The library includes spectra obtained of different sources obtained using excitation and emission filters. Name The spectrum identifier used by the unmixing algorithm. The name cannot be modified. Label The spectrum name in the spectrum plot key. The label can be edited. Color The color of the spectrum in the spectrum plot. For the SOL type spectrum, it is also the color in the composite image. Spectrum Preview Shows the spectrum selected above (click a row above the preview pane). Use this tool to pick up a pixel in an opened image and plot the spectrum at this pixel in the spectrum preview. The Results tab in the Spectral Unmixing tool palette shows the optimized fit parameters used by the software to derive the spectral unmixing results (Figure 8.2). Figure 8.2 Tool palette, Spectral unmixing tools, Results tab Table 8.2 Spectral unmixing tools, Results tab Item Description Number of Iterations The number of iterations that the algorithm used.
<strong>Living</strong> <strong>Image</strong> ® Software User’s Manual Table 8.2 Spectral unmixing tools, Results tab Item Description Number of Components The number of components unmixed. Number of Wavelengths The number of wavelength pairs used in the analysis. Number of Samples The number of pixel samples used in the analysis Lack of Fit (%PCA) The fitting residue compared to the data filtered by principal component analysis. Lack of Fit (% EXP) The fitting residue compared to the experimental data. Divergence Counter The number of divergences that occurred. Maximum Iterations The maximum number of iterations allowed. Denoise (PCA) Indicates how much of the data was filtered by principal component analysis. Normalization The normalization method used in the analysis. Non-negativity Method The non-negativity method used in the analysis. Weighting Mode The weighting method applied to the data. Column Weighting Mode Indicates if column-wise weighting was used. Row Weighting Mode Indicates if row-wise weighting was used. Click to display the spectrum plot tab. Click to display the concentration plot tab. 119
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Living Image 3.1

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