Living Image 3.1

7. Planar Spectral Image Analysis
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3. In the Analyze tab, select the emission filter
wavelengths for the analysis.
It is recommended that you do not include a
wavelength in the analysis if the signal is less than
or equal to the autoluminescent background. If
autoluminescent background is a concern, you can
create a background ROI and link it to the
measurement ROI prior to planar spectral analysis.
(For more details, see Measuring Background-
Corrected Signal, page 79.)
4. In the ROI List drop-down, select All or a particular
ROI for the analysis. If there is no measurement
ROI, draw an ROI that includes the area for
analysis. (For more details on drawing ROIs, see
Measurement ROIs, page 74.)
You only need to draw the ROI(s) on one image in
the sequence. The software copies the ROI(s) to all
other images of the sequence during the analysis.
The ROI should include as much of the light
emission from a single source as possible.
5. Choose the tissue properties:
a. In the Properties tab, make a selection from the
Tissue Properties drop-down list.
b. Choose the tissue type most representative of the
area of interest. Muscle is a good choice for a
generic tissue type.
- The software automatically sets the internal medium index of
refraction based on the selection in the Tissue Properties list.
6. Make a selection from the Source Spectrum dropdown
list.
7. Click Analyze in the Analyze tab.