Living Image 3.1

Living Image ® Software User’s Manual
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3D Reconstruction of Sources
3D Reconstruction of Bioluminescent Sources . . . . . . . . . . . . . . . . . . . . . . . 138
3D Reconstruction of Fluorescent Sources . . . . . . . . . . . . . . . . . . . . . . . . . 142
DLIT & FLIT Results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 144
3D Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 150
3D Tools - Mesh Tab . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 156
3D Tools - Volume Tab . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 158
3D Tools - Organs Tab . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 161
3D Tools - Animation Tab . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 168
Managing DLIT/FLIT Results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 173
The Living Image ® software includes algorithms for 3D reconstruction of
bioluminescent or fluorescent sources (tomographic analysis):
• Diffuse Tomography (DLIT) algorithm - For tomographic analysis of
bioluminescent sources. Analyzes a bioluminescent image sequence obtained on
the IVIS ® Imaging System 200 or IVIS Spectrum.
• Fluorescent Tomography (FLIT) algorithm - For tomographic analysis of
fluorescent sources. Analyzes a fluorescent image sequence obtained on the IVIS ®
Spectrum imaging system.
The steps to perform 3D reconstruction of bioluminescent/fluorescent sources include:
1. Acquire an image sequence.
2. Generate the surface topography (surface) of the subject.
3. Set the user-modifiable DLIT/FLIT algorithm parameters (for example, analysis
wavelengths, source spectrum, and tissue properties) and reconstruct the position,
geometry, and strength of the luminescent sources.
For more details on the DLIT/FLIT algorithm, see 3D Reconstruction of Light Sources,
page 233.
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