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Living Image 3.1

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8. Spectral Unmixing<br />

8.3 Spectral Unmixing Parameters<br />

118<br />

Table 8.1 Tool palette, spectral unmixing tools<br />

Item Description<br />

SOL A spectrum generated by the spectral unmixing algorithm.<br />

ROI A spectrum calculated for a user-selected ROI.<br />

LIB A user-selected library spectrum. The library includes spectra obtained of<br />

different sources obtained using excitation and emission filters.<br />

Name The spectrum identifier used by the unmixing algorithm. The name<br />

cannot be modified.<br />

Label The spectrum name in the spectrum plot key. The label can be edited.<br />

Color The color of the spectrum in the spectrum plot. For the SOL type<br />

spectrum, it is also the color in the composite image.<br />

Spectrum Preview Shows the spectrum selected above (click a row above the preview<br />

pane).<br />

Use this tool to pick up a pixel in an opened image and plot the spectrum<br />

at this pixel in the spectrum preview.<br />

The Results tab in the Spectral Unmixing tool palette shows the optimized fit<br />

parameters used by the software to derive the spectral unmixing results (Figure 8.2).<br />

Figure 8.2 Tool palette, Spectral unmixing tools, Results tab<br />

Table 8.2 Spectral unmixing tools, Results tab<br />

Item Description<br />

Number of Iterations The number of iterations that the algorithm used.

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