Living Image 3.1

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7. Planar Spectral Image Analysis 108 3. In the Analyze tab, select the emission filter wavelengths for the analysis. It is recommended that you do not include a wavelength in the analysis if the signal is less than or equal to the autoluminescent background. If autoluminescent background is a concern, you can create a background ROI and link it to the measurement ROI prior to planar spectral analysis. (For more details, see Measuring Background- Corrected Signal, page 79.) 4. In the ROI List drop-down, select All or a particular ROI for the analysis. If there is no measurement ROI, draw an ROI that includes the area for analysis. (For more details on drawing ROIs, see Measurement ROIs, page 74.) You only need to draw the ROI(s) on one image in the sequence. The software copies the ROI(s) to all other images of the sequence during the analysis. The ROI should include as much of the light emission from a single source as possible. 5. Choose the tissue properties: a. In the Properties tab, make a selection from the Tissue Properties drop-down list. b. Choose the tissue type most representative of the area of interest. Muscle is a good choice for a generic tissue type. - The software automatically sets the internal medium index of refraction based on the selection in the Tissue Properties list. 6. Make a selection from the Source Spectrum dropdown list. 7. Click Analyze in the Analyze tab.
Living Image ® Software User’s Manual 7.2 Planar Spectral Imaging Tools The Results tab displays the computed average depth (mm) and total flux (photon/sec) of the bioluminescent point source in the specified ROI(s). The planar spectral imaging tools are displayed in three tabs. Figure 7.1 Tool palette, planar spectral imaging tools 109
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Living Image 3.1

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