Living Image 3.1

H. 3D Reconstruction of Light Sources
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You can view tissue optical property values (μ eff ) in the Tissue Properties drop-down
list. The tissue properties are plotted as a function of wavelength. Select the tissue or
organ most representative of the source location. Muscle is a good choice for general
reconstructions in vivo.
NOTE
H.2 Algorithm Parameters & Options
Analyze Tab
Tissue and
source are
specified in the
Select the
acquisition
wavelengths
for the DLIT
analysis.
If DLIT analysis
results are
open, the 3D
tools are
available.
Default tissue optical properties and source spectrum are specified in the
Preferences box. For more details, see Appendix B, page 187.
This section explains the user-modifiable DLIT algorithm parameters and options.
Figure H.5 3D reconstruction tools, Analyze tab, DLIT (left) and FLIT (right)
Wavelengths
Active sequence
For FLIT reconstruction of fluorescent sources, you must specify the transillumination
source positions. It is recommended that you acquire images at a minimum of four
source positions. (All images are acquired using the same excitation and emission
filters.)
For DLIT reconstruction of luminescent sources, you must specify the acquisition
wavelengths for the image sequence. It is generally recommended that you acquire
image data using two to four wavelengths rather than a single wavelength so that more
information is available for the analysis.
Ideally, chose wavelengths or source positions where the signal is well above zero (not
buried in the CCD noise) and the optical property of the medium (μ eff ) exhibits a large
change. The larger the difference in μ eff , the higher the quality of information that the