Living Image 3.1

7. Planar Spectral Image Analysis
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Table 7.1 Planar spectral imaging tools & results
Item Description
Analyze Tab
Sequence Name of sequence used for the analysis.
Tissue, Source The tissue properties and source spectrum selected in the
Properties tab.
Select Filters In the Filter box, select the acquisition wavelengths for the images
in the selected sequence. To select non-adjacent wavelengths,
press and hold the Ctrl key while you click the wavelengths.
(Macintosh users, press and hold the Cmd key while you click the
wavelengths.)
ROI List A drop-down list of the ROIs in the active image.
Analyze Click to perform the spectral analysis.
Properties Tab
Tissue Properties Drop-down list of the absorption and scattering properties of
various tissues.
Internal medium Tissue index of refraction that is automatically specified when you
index of refraction select a tissue property.
Source Spectrum Drop-down list of bioluminescent sources.
Plot
Tissue Properties Click to display graphs (cm -1 vs nm) of the absorption coefficient
(μ a ), effective attenuation coefficient (μ eff ), and reduced scattering
coefficient (μ’ s ).
Source Spectrum Click to display the spectrum of the selected bioluminescent source
(intensity versus wavelength, normalized to one).
Results Tab
Spectral Results
ROI Name of the analyzed ROI.
Depth (mm) Estimated depth of the point source.
Total Flux (phot/s) Estimated total photon flux from the point source.
Plot Linear Fit Displays a graph of normalized intensity versus the effective
attenuation coefficient (μeff , the optical property of the tissue
selected in the Tissue Properties drop-down list) along with the
linear fit to these data determined by the spectral analysis code.
Plot Intensity Displays a graph of normalized intensity versus wavelength.
Intensity is normalized by the selected source spectrum and filter
transmission properties.
Export Opens a dialog box that enables you to save the results to a text file
(.txt).
Save Results
Name A drop-down list of saved results. Includes the default name for
new unsaved analysis results (SpIm_xx).
Delete Deletes the selected results.
Load Opens the selected results.
Save Saves the analysis results (results name appears in the Name dropdown
list).