Living Image 3.1

6. Image Math
6.3 Overlaying Multiple Images
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The image overlay tool provides a convenient way to view multiple reporters in one
image. You can use the image overlay tool to display multiple luminescence or
fluorescence images on one photographic image. To do this:
To coregister multiple images:
1. Acquire an image sequence using the appropriate filters for each reporter.
Alternately, create a sequence form images acquired during different sessions. (For
more details, see page 43.)
2. Open the image sequence.
Note: To view all
images in the sequence,
click the Display All
button to open each
image (overlay mode) in
a separate image
window.
3. Open one of the images and optimize the image display using the color scale Min
and Max sliders in the Image Adjust tools.
4. Select Tools→ Image Overlay for xx_SEQ on the menu bar.
- The image overlay window appears and shows the first photographic image in the
sequence.