Living Image 3.1

4. Working With Images
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3. To access controls for manually
adjusting the color rendition, click
Colorize.
Table 4.11 Colorize tools
Item Description
Colorize
Color Range The color map indicates the color range of the selected camera setup
from short to long wavelength. The two sliders determine the lower
and upper limits of the color range that is used to render color. The
parts of the color map outside the selected range are not used in the
color rendering process. By default, the entire color range is
selected.
Filter Range The wavelength range of the luminescent images in the sequence.
The two sliders determine the lower and upper end of the filter
range. Only the parts of the image that are within the selected
wavelength range are colorized. By default, the entire filter range is
selected.
Color Camera
VIS Regular camera setup that mainly renders color in the visible range.
It is similar to the color response of a commercial digital camera. NIR
fluorophores appear dark red to invisible using the VIS camera setup.
NIR A special camera setup that extends the color response into the near
infrared range. Near infrared fluorophores appear red to purple using
the NIR camera setup.
Log Scale If this option is chosen, the dynamic range of the brightness in the
image is compressed using a log scale. This improves the visibility of
dark areas in the image.
Real Color If this option is chosen, the colors are rendered using the
wavelengths that directly correspond to the camera setup. For
example, GFP appears green using real color rendering.
If this option is not chosen, the original wavelength range of the
image is modified to include the entire visible wavelength range of
the camera setup. This helps improve the color contrast.