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Living Image 3.1

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<strong>Living</strong> <strong>Image</strong> ® Software User’s Manual<br />

Manually Drawing a<br />

Measurement ROI<br />

1. Open an image or image sequence, and in the ROI tools, select Measurement ROI<br />

from the Type drop-down list.<br />

2. To specify the ROI shape:<br />

a. Click the Circle , Square , or Grid button .<br />

The grid shape is useful for drawing a grid of ROIs on an image of a microplate<br />

plate.<br />

b. On the drop-down list that appears, select the number of ROIs that you want to add to<br />

the image or the grid ROI dimensions.<br />

- The ROI(s) and intensity label(s) appear on the image. If you are working with a<br />

sequence, open an image to show the ROI intensity.<br />

3. Adjust the ROI position:<br />

a. Place the mouse pointer over the ROI. When the pointer becomes a , click the<br />

ROI.<br />

b. Drag the ROI.<br />

4. Adjust the ROI dimensions:<br />

a. Place the mouse pointer over the ROI. When the pointer becomes a<br />

ROI.<br />

, click the<br />

b. Place the mouse pointer over an ROI handle<br />

to resize the ROI.<br />

so that it becomes a . Drag the handle<br />

Note: You can also change the ROI position or size using the adjustment controls in the<br />

ROI Properties box (see Moving an ROI, page 85 and Editing ROI Dimensions,<br />

page 86).<br />

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