Living Image 3.1

Living Image ® Software User’s Manual
Setting Up a Sequence
Using the Imaging
Wizard
Table 3.4 Imaging wizard sequence options
The imaging wizard can automatically setup an image sequence for some applications
that analyze an image sequence (Table 3.3). The wizard guides you through a series of
steps, prompting you for the information that the software requires to set up the image
sequence.
Bioluminescence Acquires... Description See
Page
Image Sequence
Open Filter T Acquires an image at maximum sensitivity.
Planar Spectral T Analyze the sequence to compute the average depth and total
photon flux of a bioluminescent point source in a region of interest
(ROI).
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Spectral
Unmixing
T Analyze the sequence to determine spectral signature of different
reporters in the same image and calculate the contribution of each
reporter on each pixel in the image.
DLIT T Apply the DLIT algorithm to the sequence to reconstruct the 3D
surface topography of the subject and the position, geometry, and
strength of the luminescent sources.
Fluorescence Description See
Page
2D T T Use the data to make fluorescence measurements.
Filter Scan T Helps you determine the optimum excitation and emission filter for
a probe.
Spectral
Unmixing
T Analyze the sequence to extract the signal of one or more
fluorophores from the tissue autofluorescence.
FLIT T Apply the FLIT algorithm to the sequence to reconstruct the 3D
surface topography of the subject and the position, geometry, and
strength of the fluorescent sources.
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