Living Image 3.1

Living Image ® Software User’s Manual
9
Generating a Surface Topography
9.1 Generate the Surface Topography
Generate the Surface Topography . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 125
Managing Surfaces . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 128
A surface topography (surface) is a reconstruction of the animal surface derived from
structured light images. (For additional details on the surface topography, see page 233.)
A surface is required for point source fitting and 3D reconstruction of bioluminescent
sources (DLIT analysis) or fluorescent sources (FLIT analysis) inside a subject.
1. Load the image sequence that you want to
analyze. (For details on how to load a sequence,
see page 37).
2. In the tool palette, click Surface Topography.
3. Click Reconstruct.
- The tomography analysis box appears (Figure
9.1).
Figure 9.1 Tomography analysis box
Crop
box
Mask (purple)
4. Draw a crop box that includes a one cm margin around the subject, if possible.
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