Living Image 3.1

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© 2002-2008 Xenogen Corporation. All rights reserved. PN 124908 Xenogen Corporation 68 Elm Street Hopkinton, MA 01748 USA 1.877.522.2447 (US) 1.508.435.9500 Fax: 1.508.435.3439 E-mail: tech.support@caliperls.com www.caliperls.com Discovery in the Living Organism, IVIS Imaging System and Living Image are either registered trademarks or trademarks of Xenogen Corporation. The names of companies and products mentioned herein may be the trademarks of their respective owners. Apple, Macintosh and QuickTime are registered trademarks of Apple Computer, Inc. Microsoft, PowerPoint and Windows are either registered trademarks or trademarks of Microsoft Corporation in the United States and/or other countries. Adobe and Illustrator are either registered trademarks or trademarks of Adobe Systems Incorporated in the United States and/or other countries.
Living Image ® Software User’s Manual Contents 1 Welcome . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 1.1 What’s New In the Living Image 3.1 Software . . . . . . . . . . . . . . . . . . . . . . . . . . 1 1.2 About This Manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 1.3 Contacting Caliper Technical Support . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 2 Getting Started . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 2.1 Overview of Imaging & Image Analysis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 2.2 Starting the Living Image ® Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 2.3 Living Image Help . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 3 Initializing the System & Acquiring Images . . . . . . . . . . . . . . . . . . . . . . 11 3.1 Initializing the IVIS Imaging System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 3.2 Checking the System Temperature . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 3.3 About the IVIS Acquisition Control Panel & Auto Exposure Feature . . . . . . . . . . . . . . 13 3.4 Acquiring a Single Bioluminescence Image . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 3.5 Acquiring a Single Fluorescence Image With Epi-Illumination . . . . . . . . . . . . . . . . . 17 3.6 Acquiring a Fluorescence Image With Transillumination . . . . . . . . . . . . . . . . . . . . 19 3.7 Setting Up an Image Sequence . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21 3.8 Acquiring an Image Sequence . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30 3.9 Manually Setting the Focus . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32 3.10 Manually Saving Image Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 3.11 Exporting Image Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34 4 Working With Images . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35 4.1 Browsing & Opening Image Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35 4.2 The Tool Palette . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40 4.3 Working With an Image Sequence . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40 4.4 Creating an Image Sequence from Individual Images . . . . . . . . . . . . . . . . . . . . . . 43 4.5 Working With a Single Image . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45 4.6 Viewing Image Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49 4.7 Adjusting Image Appearance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52 4.8 Correcting or Filtering Image Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 4.9 Image Information Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56 4.10 Rendering Intensity Data in Color . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63 4.11 Viewing Transillumination Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65 4.12 Image Layout Window . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66 i
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Living Image 3.1

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