Living Image 3.1

A. IVIS Acquisition Control Panel
Table A.2 Additional IVIS ® System Controls for the IVIS Imaging System 200 Series or IVIS Spectrum
Item Description
Alignment grid Choose this option to activate a laser-generated alignment grid on the stage when the imaging
chamber door is opened. The alignment grid is set to the size of the selected FOV. The grid
automatically turns off after two minutes. If subject alignment is not completed in two minutes,
place a check mark next to Enable Alignment Grid to turn on the grid.
Note: The horizontal cross hair of the alignment grid is offset appropriately to take into account the
height entered in the Subject height box.
Focus Scan Mid Image - Choose this option in the Focus drop-down list to set the focal plane at the
maximum dorso-ventral height of the subject at the middle of the animal. This focusing method uses
the laser to scan horizontally across the middle of the subject to determine the maximum subject
height along this line. This option is well suited for animal imaging because the peak height is clearly
identified as the maximum height on the dorsal side along the mid-plane of the animal.
Note: This focusing method is not recommended for microplates or when using a high
magnification field of view (FOV A = 4.0 cm). In these situations, Manual or Subject Size focus
methods are recommended.
Transillumination Setup Choose this option to display the transillumination setup window that enables you to select the
(IVIS Spectrum only)
locations for image acquisition using bottom illumination that originates beneath the stage.
180
Table A.3 Typical field of view (FOV) settings
FOV Setting IVIS Imaging System
Lumina 100 Series
FOV (cm)
200 Series
A 4 10 3.9
B 7 15 6.5
C 10 20 13
D 12 25 19.5
E 26