Living Image 3.1

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2. Getting Started 2.3 Living Image Help 10 Menu bar (for more details, see Appendix I, page 243) Toolbar NOTE If the workstation controls the IVIS Imaging System, the acquisition control panel (for more details, see Appendix A, page 177). The Living Image ® software on the PC workstation that controls the IVIS ® Imaging System includes both the acquisition and analysis features. The Living Image software on other workstations includes only the analysis features. Macintosh users have access to only the analysis features of the Living Image software. There are several ways to obtain help on the software features: • To view a tooltip about a button function, put the mouse cursor over the button. • To view a brief description about an item in the user interface, click the toolbar button, then click the item. Press F1 or select Help → User Guide on the menu bar to display the Living Image 3.1 Software User’s Manual (.pdf).
Living Image ® Software User’s Manual 3 Initializing the System & Acquiring Images 3.1 Initializing the IVIS Imaging System Initializing the IVIS Imaging System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 Checking the System Temperature . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 About the IVIS Acquisition Control Panel & Auto Exposure Feature . . . . . . . . . . . 13 Acquiring a Single Bioluminescence Image . . . . . . . . . . . . . . . . . . . . . . . . . 14 Acquiring a Single Fluorescence Image With Epi-Illumination . . . . . . . . . . . . . . . 17 Acquiring a Fluorescence Image With Transillumination . . . . . . . . . . . . . . . . . . 19 Setting Up an Image Sequence . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21 Acquiring an Image Sequence . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30 Manually Setting the Focus . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32 Manually Saving Image Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 Exporting Image Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34 The IVIS ® Imaging System is ready for image acquisition after the system is initialized and the operating (demand) temperature of the CCD camera is reached (locked). The Living Image ® software on the PC workstation that controls the IVIS Imaging System includes both the acquisition and analysis features. The Living Image software on other workstations includes only the analysis features. The imaging system must be initialized each time the Living Image ® software is started or if the power has been cycled to the imaging chamber or the camera controller (a component of some IVIS systems). The initialization procedure moves every motordriven component in the system (for example, stage and lens) to a home position, resets all electronics and controllers, and restores all software variables to the default settings. Initialization may be useful in error situations. For further details on instrument operation, see the hardware manual for your IVIS Imaging System. To initialize the imaging system: 1. Start the Living Image software (double-click the icon on the desktop). 2. In the control panel that appears, click Initialize. - You will hear the motors move. 11
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Living Image 3.1

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