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Living Image 3.1

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<strong>Living</strong> <strong>Image</strong> ® Software User’s Manual<br />

<strong>3.1</strong>0 Manually Saving <strong>Image</strong> Data<br />

2. To mark the center of the camera in the window, put a check mark next to Display<br />

CCD Center.<br />

3. Select the size of the step increment that the stage moves: Coarse, Normal, or<br />

Fine.<br />

4. Click Up or Down to move the stage and change the focus.<br />

5. If necessary, select another F/stop setting from the drop-down list and adjust the<br />

light level using the arrows.<br />

6. Click Update to apply the settings.<br />

- The resulting focal plane (cm above the stage) is automatically entered in the<br />

Subject height box.<br />

7. Click OK when the image is focused.<br />

When you acquire the first image(s) of a session, the autosave feature prompts you to<br />

choose the folder where you want to save image data. During the session, all images<br />

will be saved to this folder. You can change the autosave folder at any time (select<br />

Acquisition → Auto-Save To on the menu bar). If you do not want to use the autosave<br />

feature, you can manually save data.<br />

1. Turn off the autosave feature: select Acquisition on the menu bar and remove the<br />

check mark next to Auto Save.<br />

2. After you acquire an image or image sequence, click the Save button .<br />

Alternatively, select File → Save on the menu bar.<br />

3. In the dialog box that appears, select a directory of interest and click OK.<br />

NOTE<br />

The software automatically includes the user ID, and a date and time stamp with the<br />

data.<br />

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