Living Image 3.1

11. 3D Reconstruction of Sources
11.2 3D Reconstruction of Fluorescent Sources
Set Up & Acquire an
Image Sequence on the
IVIS ® Spectrum
Generate the Surface
Topography
Set the FLIT
Parameters &
Reconstruct the
Sources
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Use the imaging wizard to setup the image sequence required for FLIT analysis. (For
more details on the imaging wizard, see page 23.) If you plan to manually set up the
sequence, Table 11.2 shows the recommended image sequence. Acquire the images
using transillumination on the IVIS Spectrum using the same excitation and emission
filters from at least four source locations.
Table 11.2 Example image sequence for FLIT analysis
Image Type Source Location
First location Subsequent locations
Photographic ✓ Use previous photo
Structured light ✓
Fluorescent ✓ ✓
For instructions, see page 137.
After the surface is generated, the 3D reconstruction of the light sources can proceed.
1. In the tool palette, click FLIT 3D Reconstruction.
Analyze tab
Source 01 corresponds to the first
image of the sequence, source 02
corresponds to the second image,
and so on
2. In the Properties tab, make a selection from the Tissue Properties drop-down list.
“Muscle” is usually the best choice of tissue properties for general in vivo
reconstructions.
Note: The internal medium index of refraction is automatically entered when you
select a tissue.