Living Image 3.1

Living Image ® Software User’s Manual
Parameters Tab
wavelength data adds to the analysis. The recommended wavelengths are 580-640 nm for
tissue and 560-620 nm for the Xenogen XPM-2 tissue phantom.
Minimum Radiance
The minimum radiance determines the lower radiance [photons/sec/cm 2/sr] threshold of
the data to be used in the DLIT/FLIT analysis.
The software automatically computes a default minimum radiance value and this is the
recommended starting point for an analysis. If too much noise is included in the
analysis, increase the minimum radiance value.
An optimum minimum radiance value can be evaluated by viewing the image data in
photon units (photons/sec/cm 2/sr) and adjusting the color bar Min to be above the level
of noise in the image.
Figure H.6 3D reconstruction tools, Parameters tab, DLIT (left) and FLIT (right)
Angle Limit
The angle limit refers to the angle between the object surface normal and the optical
axis. The optical axis can be considered to be a line perpendicular to the stage. The
surface normal is a line perpendicular to a plane tangent to the surface point. For
example, in a dorsal view of a mouse, the highest point on its back would have a normal
line perpendicular to the stage. In this case the angle is zero. The side of a mouse
abdomen would have a normal line parallel to the stage, so the angle here would be close
to 90° .
The software uses luminescent image data for surface elements that are less than the
angle limit. The default angle limit setting is 70° for the IVIS ® Imaging System 200
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