Living Image 3.1
Living Image 3.1
Living Image 3.1
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<strong>Living</strong> <strong>Image</strong> ® Software User’s Manual<br />
Index<br />
Numerics<br />
3D fluorescent sources 142–143<br />
3D image<br />
rotate or move 152<br />
3D luminescent sources 138–141<br />
3D perspective 155<br />
3D reconstruction<br />
fluorescent sources 142–143<br />
parameters & options 238–241<br />
3D reconstruction results 144–146<br />
3D tools 148–173<br />
Animation tab 168–169<br />
Mesh tab 156–157<br />
Organs tab 161–162<br />
Volume tab 158–159<br />
A<br />
acquire<br />
image sequence 30–31<br />
single bioluminescence image 14–17<br />
single fluorescence image (epi-illumination) 17–18<br />
single fluorescence image (trans-illumination)<br />
19–21<br />
adaptive fluorescent background subtraction 221<br />
adjusting image appearance 52<br />
angle limit 239<br />
animation 168–173<br />
custom 171–172<br />
edit an animation setup 172–173<br />
preset 170–171<br />
auto exposure feature 13<br />
autofluorescence 79, 215<br />
miscellaneous material 217–219<br />
See tissue autofluorescence.<br />
subtract using background filters 223–224<br />
well plate 216–217<br />
autoluminescence 79<br />
automatically draw ROIs 76–77<br />
average background ROI 70, 79<br />
B<br />
background<br />
adaptive fluorescent background subtraction 221<br />
fluorescent 215–221<br />
light on sample 204–206<br />
tissue autofluorescence 223–224<br />
background light<br />
from sample 206–208<br />
on sample 204–206<br />
background-corrected signal 79–81<br />
band gap 212<br />
bandpass filter 211<br />
binning 55, 56, 192–194<br />
bioluminescence image 14–17<br />
browse image data 35–37<br />
browser 36<br />
C<br />
Caliper Corporation<br />
technical support 3<br />
cascade images 48<br />
color table 197<br />
colorize data 63–64<br />
composite image 98–101<br />
control panel 13, 177–180<br />
conventions 2<br />
copy<br />
ROI measurements 92<br />
correction/filtering tools<br />
binning 55<br />
cosmic correction 55<br />
dark background subtraction 55<br />
flat field correction 55<br />
smoothing 55<br />
cosmic correction 55<br />
cosmic ray corrections 202<br />
counts 199<br />
crop box 62<br />
D<br />
dark background subtraction 55<br />
dark charge 204<br />
dark current 203<br />
data<br />
graphic image 197<br />
scientific image 197<br />
detection efficiency 191<br />
detection sensitivity<br />
247
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